Detailed information of TA system TA045258

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4150735..4151342
• Replicon: chromosome
• Accession: NZ_CP006569
• Organism: Sodalis praecaptivus strain HS1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: W0HXM1
• Locus tag: SANT_RS18530
• Protein ID: WP_025423733.1
• Coordinates: 4150735..4151046 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: SANT_RS18535
• Protein ID: WP_025423734.1
• Coordinates: 4151046..4151342 (+)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SANT_RS18515	4147500..4149347	+	1848	WP_025423731.1	RNA polymerase sigma factor RpoD	-
SANT_RS18520	4149462..4149962	-	501	WP_025423732.1	G/U mismatch-specific DNA glycosylase	-
SANT_RS18530	4150735..4151046	+	312	WP_025423733.1	toxin HigB-2	Toxin
SANT_RS18535	4151046..4151342	+	297	WP_025423734.1	helix-turn-helix domain-containing protein	Antitoxin
SANT_RS18540	4153312..4153668	+	357	WP_148296325.1	hypothetical protein	-
SANT_RS18545	4154029..4155330	-	1302	WP_038668790.1	MHS family MFS transporter	-
SANT_RS18550	4155334..4155669	-	336	WP_025423737.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-87)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12115.77 Da
• Isoelectric Point: 7.2024

>T45258 WP_025423733.1 NZ_CP006569:4150735-4151046 [Sodalis praecaptivus]
MLFIETHIFTEDVKELLNDDEYREFQQFMADNPDWGDVIQNTGGLRKIRWAARGKGKRGGVRVIYYHKVTESQIRLLLIY
KKGVQDDLSEGDKRQLRALTEGW

Nucleotide

• Length: 312 bp

>T45258 NZ_CP006569:4150735-4151046 [Sodalis praecaptivus]
ATGCTGTTTATCGAAACCCATATTTTCACCGAAGATGTAAAGGAACTATTGAACGATGACGAATACCGTGAGTTTCAGCA
GTTCATGGCAGATAACCCCGATTGGGGGGATGTAATTCAAAATACTGGCGGGCTGCGTAAAATCCGTTGGGCGGCCAGAG
GCAAGGGCAAACGTGGAGGTGTCCGAGTGATTTACTATCACAAGGTCACTGAATCACAAATCAGGCTGTTGCTGATCTAC
AAAAAAGGCGTCCAGGATGATTTATCCGAGGGCGACAAGCGCCAGTTAAGGGCGTTAACCGAGGGCTGGTAA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 10925.77 Da
• Isoelectric Point: 10.6079

>AT45258 WP_025423734.1 NZ_CP006569:4151046-4151342 [Sodalis praecaptivus]
MEKKLFERLKESMMQMNEIIEGSREPSRETNVIAVKVKAIRTATGLSQSGFAKLISVNVDTLKNWEQGRREPTGPAKALL
KAIERDPLHVIPALSAKP

Nucleotide

• Length: 297 bp

>AT45258 NZ_CP006569:4151046-4151342 [Sodalis praecaptivus]
ATGGAAAAAAAACTATTTGAGCGTCTGAAAGAAAGTATGATGCAAATGAATGAAATCATCGAGGGTTCCCGCGAACCCTC
TCGCGAGACAAATGTTATCGCCGTTAAGGTTAAAGCTATCAGGACGGCTACAGGGTTGTCACAAAGCGGGTTCGCTAAGC
TGATTTCGGTCAATGTTGATACGTTAAAGAATTGGGAACAGGGCAGACGTGAACCCACTGGGCCAGCTAAGGCTCTTTTG
AAAGCCATTGAGCGCGACCCGTTACACGTGATCCCAGCGCTATCAGCTAAACCGTGA

Structures

Toxin

• AlphaFold DB: W0HXM1