Detailed information of TA system TA045254

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 2924708..2925315
• Replicon: chromosome
• Accession: NZ_CP006569
• Organism: Sodalis praecaptivus strain HS1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: W0HZC5
• Locus tag: SANT_RS12875
• Protein ID: WP_025422708.1
• Coordinates: 2924708..2925019 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: SANT_RS12880
• Protein ID: WP_025422709.1
• Coordinates: 2925019..2925315 (+)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SANT_RS24160	2920042..2920302	+	261	WP_148296287.1	hypothetical protein	-
SANT_RS23375	2921841..2922076	-	236	Protein_2515	helix-turn-helix domain-containing protein	-
SANT_RS12865	2922185..2922436	+	252	WP_025422706.1	helix-turn-helix domain-containing protein	-
SANT_RS12870	2922563..2923465	+	903	WP_025422707.1	Rpn family recombination-promoting nuclease/putative transposase	-
SANT_RS24500	2923467..2923619	-	153	WP_158500159.1	hypothetical protein	-
SANT_RS12875	2924708..2925019	+	312	WP_025422708.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
SANT_RS12880	2925019..2925315	+	297	WP_025422709.1	helix-turn-helix domain-containing protein	Antitoxin
SANT_RS12885	2925961..2926293	+	333	WP_025422710.1	type II toxin-antitoxin system RelE/ParE family toxin	-
SANT_RS12890	2926319..2926609	+	291	WP_025422711.1	helix-turn-helix domain-containing protein	-
SANT_RS12895	2927144..2927593	-	450	WP_025422712.1	hypothetical protein	-
SANT_RS12900	2927702..2929378	-	1677	WP_025422713.1	hypothetical protein	-
SANT_RS12905	2929375..2929914	-	540	WP_025422714.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2913026..2953753	40727

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (43-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-87)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12184.83 Da
• Isoelectric Point: 5.9033

>T45254 WP_025422708.1 NZ_CP006569:2924708-2925019 [Sodalis praecaptivus]
MLFIETHIFTEDVKELLNDDEYREFQQFMADNPDWGDVIQNTGGLRKIRWAAKGKGKRGGVRVIYYYKVTESQIRLLLIY
KKGVQDDLSEDDKRQLRALNEGW

Nucleotide

• Length: 312 bp

>T45254 NZ_CP006569:2924708-2925019 [Sodalis praecaptivus]
ATGCTGTTTATCGAAACCCATATTTTCACCGAAGATGTAAAGGAACTATTGAACGATGACGAATATCGCGAGTTTCAGCA
GTTCATGGCCGATAACCCCGATTGGGGGGATGTAATCCAAAATACTGGAGGACTGCGTAAAATCCGCTGGGCGGCCAAAG
GCAAGGGCAAGCGTGGGGGCGTCCGTGTGATTTACTATTACAAGGTCACCGAATCACAAATCAGGCTGTTGCTGATCTAC
AAAAAAGGCGTCCAGGATGATTTATCCGAGGACGACAAGCGCCAGTTAAGGGCGTTAAATGAGGGCTGGTAA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 10885.60 Da
• Isoelectric Point: 10.4846

>AT45254 WP_025422709.1 NZ_CP006569:2925019-2925315 [Sodalis praecaptivus]
MEQKLFERLKESMTQMNEIIEGSREPSRETNVIAVKVKAIRTATGLSQSGFAKLISVNVDTLKNWEQGRREPTGPAKALL
KAIERDPLHVIPALSAKS

Nucleotide

• Length: 297 bp

>AT45254 NZ_CP006569:2925019-2925315 [Sodalis praecaptivus]
ATGGAACAAAAACTGTTTGAGCGTCTGAAAGAAAGTATGACGCAAATGAATGAAATCATCGAAGGTTCCCGCGAACCCTC
GCGAGAAACAAACGTTATTGCTGTTAAGGTTAAAGCTATCAGAACGGCTACAGGGTTGTCACAAAGCGGGTTCGCTAAGC
TGATTTCGGTCAATGTTGATACGTTAAAGAATTGGGAACAGGGCAGACGTGAACCCACTGGCCCAGCTAAGGCTCTTTTG
AAAGCCATTGAGCGCGACCCGTTACACGTGATCCCAGCGCTATCAGCTAAATCTTGA

Structures

Toxin

• AlphaFold DB: W0HZC5