Detailed information of TA system TA045240

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 11621..12216
• Replicon: plasmid pAU20d
• Accession: NZ_CP006371
• Organism: Aureimonas sp. AU20

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: M673_RS23860
• Protein ID: WP_082640066.1
• Coordinates: 11935..12216 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A0S2EWK4
• Locus tag: M673_RS22485
• Protein ID: WP_061978987.1
• Coordinates: 11621..11926 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M673_RS22475	10286..11050	-	765	WP_082640065.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
M673_RS22480	11007..11372	-	366	WP_061978986.1	hypothetical protein	-
M673_RS22485	11621..11926	-	306	WP_061978987.1	HigA family addiction module antidote protein	Antitoxin
M673_RS23860	11935..12216	-	282	WP_082640066.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
M673_RS23865	12454..13002	-	549	WP_082640067.1	PAS domain-containing protein	-
M673_RS22495	13174..13536	+	363	WP_061978989.1	helix-turn-helix domain-containing protein	-
M673_RS22500	13833..14297	-	465	WP_082640068.1	NUDIX hydrolase	-
M673_RS23870	14546..14689	+	144	WP_082640069.1	DUF1127 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..101355	101355

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (20-69)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10396.63 Da
• Isoelectric Point: 6.4789

>T45240 WP_082640066.1 NZ_CP006371:c12216-11935 [Aureimonas sp. AU20]
VIRAYRGKHTEAVASGESPKGFPGDLLRASQRKLAMLNAAAALEDLRAPPGNRLEALHGDREGQHSIRINDQWRICFHWT
DAGPEDVEIVDYY

Nucleotide

• Length: 282 bp

>T45240 NZ_CP006371:c12216-11935 [Aureimonas sp. AU20]
GTGATCCGAGCCTATCGAGGCAAGCATACGGAGGCGGTCGCTTCGGGAGAGTCACCGAAGGGTTTCCCAGGCGATCTCCT
CAGGGCTTCCCAGCGCAAGCTTGCCATGCTGAACGCTGCCGCTGCTCTCGAGGATCTGCGCGCCCCGCCGGGCAACCGGC
TGGAAGCGCTCCATGGAGACCGCGAGGGCCAGCATTCCATACGGATCAACGATCAGTGGCGCATCTGCTTCCACTGGACG
GATGCCGGACCTGAAGACGTGGAAATCGTGGATTATTATTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11461.25 Da
• Isoelectric Point: 5.8787

>AT45240 WP_061978987.1 NZ_CP006371:c11926-11621 [Aureimonas sp. AU20]
MLANMLPPIHPGEILREEFLIPLDLTPYALAKAIDVPRTRIERLAREETPVTTDTALRLARYFGTSAEFWTSLQATFELR
TEGEAKREALLKIQPRQNVAA

Nucleotide

• Length: 306 bp

>AT45240 NZ_CP006371:c11926-11621 [Aureimonas sp. AU20]
ATGCTCGCGAACATGCTGCCGCCGATCCATCCCGGCGAAATTCTGCGGGAGGAGTTCCTGATCCCGCTCGATCTGACGCC
TTATGCTCTCGCCAAGGCGATCGACGTTCCACGCACGCGGATCGAACGTCTGGCGCGTGAGGAAACCCCTGTCACGACCG
ACACCGCCCTCAGGCTTGCTCGCTATTTCGGAACGTCTGCCGAGTTCTGGACGAGCCTACAAGCGACGTTCGAGCTTAGA
ACGGAAGGCGAGGCAAAGCGCGAAGCGCTGCTGAAGATCCAGCCGCGCCAAAACGTGGCGGCTTGA

Structures

Antitoxin

• AlphaFold DB: A0A0S2EWK4