Detailed information of TA system TA045232

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 61602..62028
• Replicon: plasmid pO145-13516
• Accession: NZ_CP006263
• Organism: Escherichia coli O145:H28 str. RM13516

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: ECRM13516_RS27810
• Protein ID: WP_001312861.1
• Coordinates: 61602..61760 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	61804..62028 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECRM13516_RS27770	56651..56878	-	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-
ECRM13516_RS27775	56973..57659	-	687	WP_025380718.1	PAS domain-containing protein	-
ECRM13516_RS27780	57850..58233	-	384	WP_000124981.1	relaxosome protein TraM	-
ECRM13516_RS27785	58510..59157	+	648	WP_122994606.1	transglycosylase SLT domain-containing protein	-
ECRM13516_RS27790	59454..60275	-	822	WP_001234426.1	DUF945 domain-containing protein	-
ECRM13516_RS27795	60393..60680	-	288	WP_000107535.1	hypothetical protein	-
ECRM13516_RS27810	61602..61760	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	61804..62028	-	225	NuclAT_0	-	Antitoxin
-	61804..62028	-	225	NuclAT_0	-	Antitoxin
-	61804..62028	-	225	NuclAT_0	-	Antitoxin
-	61804..62028	-	225	NuclAT_0	-	Antitoxin
ECRM13516_RS32825	61840..62028	+	189	WP_001299721.1	hypothetical protein	-
ECRM13516_RS27820	62040..62759	-	720	WP_001276238.1	plasmid SOS inhibition protein A	-
ECRM13516_RS27825	62756..63190	-	435	WP_025380720.1	conjugation system SOS inhibitor PsiB	-
ECRM13516_RS30915	63245..63442	-	198	Protein_72	hypothetical protein	-
ECRM13516_RS27830	63470..63703	-	234	WP_025380721.1	DUF905 family protein	-
ECRM13516_RS27835	63766..64305	-	540	WP_025380722.1	single-stranded DNA-binding protein	-
ECRM13516_RS33045	64331..64537	-	207	WP_000547944.1	hypothetical protein	-
ECRM13516_RS27845	65146..65337	-	192	WP_001027500.1	hypothetical protein	-
ECRM13516_RS27850	65334..65798	-	465	WP_044697612.1	DUF1380 family protein	-
ECRM13516_RS27855	65798..66100	-	303	WP_072128865.1	antirestriction protein	-
ECRM13516_RS33050	66641..66958	-	318	Protein_79	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	stcE / exeE / exeG / espP / hlyC / hlyA / hlyB / hlyD	1..98066	98066

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T45232 WP_001312861.1 NZ_CP006263:c61760-61602 [Escherichia coli O145:H28 str. RM13516]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T45232 NZ_CP006263:c61760-61602 [Escherichia coli O145:H28 str. RM13516]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT45232 NZ_CP006263:c62028-61804 [Escherichia coli O145:H28 str. RM13516]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin