Detailed information of TA system TA045224

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsRA (relBE)/MqsR-MqsA
• Location: 3753455..3754148
• Replicon: chromosome
• Accession: NZ_CP006262
• Organism: Escherichia coli O145:H28 str. RM13516

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: S1EZG2
• Locus tag: ECRM13516_RS19085
• Protein ID: WP_000415584.1
• Coordinates: 3753852..3754148 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: mqsA
• Uniprot ID: S1EBV2
• Locus tag: ECRM13516_RS19080
• Protein ID: WP_000650107.1
• Coordinates: 3753455..3753850 (-)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECRM13516_RS19070	3749319..3751577	-	2259	WP_001281878.1	DNA topoisomerase IV subunit A	-
ECRM13516_RS19075	3751715..3753322	-	1608	WP_001400746.1	ABC transporter substrate-binding protein	-
ECRM13516_RS19080	3753455..3753850	-	396	WP_000650107.1	type II toxin-antitoxin system antitoxin MqsA	Antitoxin
ECRM13516_RS19085	3753852..3754148	-	297	WP_000415584.1	type II toxin-antitoxin system toxin MqsR	Toxin
ECRM13516_RS19090	3754353..3754835	-	483	WP_000183497.1	GyrI-like domain-containing protein	-
ECRM13516_RS19095	3754888..3755280	-	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
ECRM13516_RS19100	3755432..3756091	+	660	WP_001221502.1	two-component system response regulator QseB	-
ECRM13516_RS19105	3756088..3757437	+	1350	WP_000673396.1	two-component system sensor histidine kinase QseC	-
ECRM13516_RS19110	3757519..3757815	-	297	WP_000912118.1	DUF2645 family protein	-
ECRM13516_RS19115	3758173..3758754	+	582	WP_000065430.1	NADPH:quinone oxidoreductase MdaB	-
ECRM13516_RS19120	3758785..3759099	+	315	WP_000958598.1	antibiotic biosynthesis monooxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (5-97)

Antitoxin

• Zinc finger, MqsA-type (3-47)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11231.96 Da
• Isoelectric Point: 8.9070

>T45224 WP_000415584.1 NZ_CP006262:c3754148-3753852 [Escherichia coli O145:H28 str. RM13516]
MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCNVIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQV
YLKITVIHDVLIVSFKEK

Nucleotide

• Length: 297 bp

>T45224 NZ_CP006262:c3754148-3753852 [Escherichia coli O145:H28 str. RM13516]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCACTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGACTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14703.10 Da
• Isoelectric Point: 9.2136

>AT45224 WP_000650107.1 NZ_CP006262:c3753850-3753455 [Escherichia coli O145:H28 str. RM13516]
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR

Nucleotide

• Length: 396 bp

>AT45224 NZ_CP006262:c3753850-3753455 [Escherichia coli O145:H28 str. RM13516]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACACAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Structures

Toxin

• PDB: 3HI2

Antitoxin

• PDB: 3HI2
• PDB: 3GA8
• PDB: 3O9X
• PDB: 2KZ8
• PDB: 3FMY
• PDB: 3GN5
• AlphaFold DB: A0A7U9LU65