Detailed information of TA system TA045221

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3392250..3392977
• Replicon: chromosome
• Accession: NZ_CP006262
• Organism: Escherichia coli O145:H28 str. RM13516

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q3YYE6
• Locus tag: ECRM13516_RS17355
• Protein ID: WP_000547563.1
• Coordinates: 3392666..3392977 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: ECRM13516_RS17350
• Protein ID: WP_000126294.1
• Coordinates: 3392250..3392669 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECRM13516_RS17330	3387299..3387826	-	528	WP_001078777.1	electron transport protein HydN	-
ECRM13516_RS17335	3387975..3388985	-	1011	WP_032161993.1	DNA-binding transcriptional regulator AscG	-
ECRM13516_RS17340	3389246..3390703	+	1458	WP_001107816.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
ECRM13516_RS17345	3390712..3392136	+	1425	WP_025380565.1	6-phospho-beta-glucosidase AscB	-
ECRM13516_RS17350	3392250..3392669	-	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
ECRM13516_RS17355	3392666..3392977	-	312	WP_000547563.1	type II toxin-antitoxin system HigB family toxin	Toxin
ECRM13516_RS17360	3393139..3393894	-	756	WP_001400787.1	hypothetical protein	-
ECRM13516_RS17365	3393937..3394404	-	468	WP_000132960.1	hydrogenase maturation peptidase HycI	-
ECRM13516_RS17370	3394397..3394807	-	411	WP_001291921.1	formate hydrogenlyase assembly protein HycH	-
ECRM13516_RS17375	3394804..3395571	-	768	WP_000067400.1	formate hydrogenlyase subunit HycG	-
ECRM13516_RS17380	3395571..3396113	-	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
ECRM13516_RS17385	3396123..3397832	-	1710	WP_001288147.1	formate hydrogenlyase subunit HycE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12398.11 Da
• Isoelectric Point: 9.7105

>T45221 WP_000547563.1 NZ_CP006262:c3392977-3392666 [Escherichia coli O145:H28 str. RM13516]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T45221 NZ_CP006262:c3392977-3392666 [Escherichia coli O145:H28 str. RM13516]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACGCCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT45221 WP_000126294.1 NZ_CP006262:c3392669-3392250 [Escherichia coli O145:H28 str. RM13516]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT45221 NZ_CP006262:c3392669-3392250 [Escherichia coli O145:H28 str. RM13516]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAGTATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCGGTT
GGCGTGGCGTTACTTCGCACGTTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACAATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A7U9QA22