Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2544698..2545200 Replicon chromosome
Accession NZ_CP006262
Organism Escherichia coli O145:H28 str. RM13516

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag ECRM13516_RS13155 Protein ID WP_000767829.1
Coordinates 2544698..2544952 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag ECRM13516_RS13160 Protein ID WP_001259255.1
Coordinates 2544949..2545200 (-) Length 84 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECRM13516_RS13130 2539713..2539940 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
ECRM13516_RS13135 2539954..2541012 - 1059 WP_000492332.1 transport protein YeeE -
ECRM13516_RS13140 2541191..2542549 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
ECRM13516_RS32310 2542539..2542601 - 63 WP_010723108.1 membrane protein YoeI -
ECRM13516_RS13145 2542816..2543745 - 930 WP_000803351.1 LysR family transcriptional regulator -
ECRM13516_RS13150 2543791..2544615 - 825 WP_000754755.1 SDR family oxidoreductase -
ECRM13516_RS13155 2544698..2544952 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
ECRM13516_RS13160 2544949..2545200 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
ECRM13516_RS29780 2545484..2545534 + 51 WP_001364200.1 his operon leader peptide -
ECRM13516_RS13165 2545680..2546579 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
ECRM13516_RS13170 2546585..2547889 + 1305 WP_001400693.1 histidinol dehydrogenase -
ECRM13516_RS13175 2547886..2548956 + 1071 WP_001099213.1 histidinol-phosphate transaminase -
ECRM13516_RS13180 2548956..2550023 + 1068 WP_000080083.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(2-84)

Antitoxin

(1-73)


Sequences


Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T45220 WP_000767829.1 NZ_CP006262:c2544952-2544698 [Escherichia coli O145:H28 str. RM13516]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T45220 NZ_CP006262:c2544952-2544698 [Escherichia coli O145:H28 str. RM13516]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT45220 WP_001259255.1 NZ_CP006262:c2545200-2544949 [Escherichia coli O145:H28 str. RM13516]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT45220 NZ_CP006262:c2545200-2544949 [Escherichia coli O145:H28 str. RM13516]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q


Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References