Detailed information of TA system TA045204

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 1077348..1077573
• Replicon: chromosome
• Accession: NZ_CP006262
• Organism: Escherichia coli O145:H28 str. RM13516

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: S1ELB5
• Locus tag: ECRM13516_RS05190
• Protein ID: WP_000813254.1
• Coordinates: 1077418..1077573 (+)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	1077348..1077406 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECRM13516_RS05165	1072608..1073714	+	1107	WP_025380294.1	hypothetical protein	-
ECRM13516_RS05170	1073721..1074467	+	747	WP_000788759.1	ATP-binding protein	-
ECRM13516_RS05175	1074489..1075259	+	771	WP_000451007.1	DUF1627 domain-containing protein	-
ECRM13516_RS05180	1075275..1075688	+	414	WP_001151233.1	DUF977 family protein	-
ECRM13516_RS05185	1076040..1076813	-	774	WP_000160654.1	alpha/beta hydrolase	-
-	1077348..1077406	-	59	-	-	Antitoxin
ECRM13516_RS05190	1077418..1077573	+	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	Toxin
ECRM13516_RS31170	1077741..1078019	+	279	WP_001341388.1	hypothetical protein	-
ECRM13516_RS05200	1078021..1079070	+	1050	WP_025380295.1	DUF968 domain-containing protein	-
ECRM13516_RS05205	1079083..1079454	+	372	WP_001217436.1	RusA family crossover junction endodeoxyribonuclease	-
ECRM13516_RS05210	1079444..1079815	+	372	WP_000090265.1	antitermination protein	-
ECRM13516_RS05215	1079967..1080785	+	819	WP_000265267.1	CPBP family intramembrane metalloprotease	-
ECRM13516_RS05220	1081072..1081269	+	198	WP_000917737.1	hypothetical protein	-
ECRM13516_RS05225	1081407..1082120	+	714	WP_000261909.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5736.89 Da
• Isoelectric Point: 6.1531

>T45204 WP_000813254.1 NZ_CP006262:1077418-1077573 [Escherichia coli O145:H28 str. RM13516]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T45204 NZ_CP006262:1077418-1077573 [Escherichia coli O145:H28 str. RM13516]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAATCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT45204 NZ_CP006262:c1077406-1077348 [Escherichia coli O145:H28 str. RM13516]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTAGATTTGCCGTTCATAGATTGAGCCTCA

Structures

Toxin

• AlphaFold DB: A0A4P7TMW0

Antitoxin