Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | higBA (relBE)/COG4683-HTH_37 |
| Location | 49571..50228 | Replicon | plasmid pRM13514 |
| Accession | NZ_CP006029 | ||
| Organism | Escherichia coli O145:H28 str. RM13514 | ||
Toxin (Protein)
| Gene name | higB | Uniprot ID | U3PDC3 |
| Locus tag | ECRM13514_RS29380 | Protein ID | WP_000270043.1 |
| Coordinates | 49571..49921 (+) | Length | 117 a.a. |
Antitoxin (Protein)
| Gene name | higA | Uniprot ID | - |
| Locus tag | ECRM13514_RS29385 | Protein ID | WP_000124640.1 |
| Coordinates | 49926..50228 (+) | Length | 101 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| ECRM13514_RS29330 | 44640..44819 | - | 180 | Protein_55 | helix-turn-helix transcriptional regulator | - |
| ECRM13514_RS29335 | 45126..45830 | + | 705 | WP_001067855.1 | IS6-like element IS26 family transposase | - |
| ECRM13514_RS29340 | 46045..46542 | - | 498 | WP_000062185.1 | hypothetical protein | - |
| ECRM13514_RS29345 | 46545..47033 | - | 489 | WP_001273096.1 | DUF1643 domain-containing protein | - |
| ECRM13514_RS29350 | 47130..47465 | + | 336 | WP_000683476.1 | hypothetical protein | - |
| ECRM13514_RS29355 | 47480..47950 | - | 471 | WP_001281821.1 | hypothetical protein | - |
| ECRM13514_RS33435 | 47943..48314 | - | 372 | WP_000516916.1 | hypothetical protein | - |
| ECRM13514_RS33440 | 48325..48519 | - | 195 | WP_000343597.1 | hypothetical protein | - |
| ECRM13514_RS29375 | 48860..49408 | - | 549 | WP_001061574.1 | transcriptional regulator | - |
| ECRM13514_RS29380 | 49571..49921 | + | 351 | WP_000270043.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| ECRM13514_RS29385 | 49926..50228 | + | 303 | WP_000124640.1 | XRE family transcriptional regulator | Antitoxin |
| ECRM13514_RS29390 | 50255..50548 | - | 294 | WP_001239997.1 | chromosome segregation protein ParM | - |
| ECRM13514_RS29395 | 50636..50908 | - | 273 | WP_001043047.1 | HU family DNA-binding protein | - |
| ECRM13514_RS29400 | 50966..51493 | - | 528 | WP_001236377.1 | thermonuclease family protein | - |
| ECRM13514_RS29410 | 51724..52581 | - | 858 | WP_001167032.1 | hypothetical protein | - |
| ECRM13514_RS29415 | 52568..52798 | - | 231 | WP_000972663.1 | hypothetical protein | - |
| ECRM13514_RS29420 | 52798..53316 | - | 519 | WP_000210756.1 | nitrite reductase | - |
| ECRM13514_RS29425 | 53313..53759 | - | 447 | WP_000919345.1 | Fe3+-siderophore ABC transporter permease | - |
| ECRM13514_RS29430 | 53759..54118 | - | 360 | WP_000422768.1 | hypothetical protein | - |
| ECRM13514_RS29435 | 54175..54603 | - | 429 | WP_000591074.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Mobilizable plasmid | sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) / floR | - | 1..64561 | 64561 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 117 a.a. Molecular weight: 13328.21 Da Isoelectric Point: 5.2421
>T45193 WP_000270043.1 NZ_CP006029:49571-49921 [Escherichia coli O145:H28 str. RM13514]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE
Download Length: 351 bp
>T45193 NZ_CP006029:49571-49921 [Escherichia coli O145:H28 str. RM13514]
ATGTGGGTCATCGAGACAACCGACACCTTTGATGAGTGGTTCGATGCTCTGGATGATACCGATAGAGCAAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGCCCCATGCTGTCGAGGCCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTTCGGGTCCAAAGCAAAGGAGATCCTATCAGAGCGTTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGTGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA
ATGTGGGTCATCGAGACAACCGACACCTTTGATGAGTGGTTCGATGCTCTGGATGATACCGATAGAGCAAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGCCCCATGCTGTCGAGGCCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTTCGGGTCCAAAGCAAAGGAGATCCTATCAGAGCGTTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGTGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA
Antitoxin
Download Length: 101 a.a. Molecular weight: 11006.73 Da Isoelectric Point: 7.2036
>AT45193 WP_000124640.1 NZ_CP006029:49926-50228 [Escherichia coli O145:H28 str. RM13514]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV
Download Length: 303 bp
>AT45193 NZ_CP006029:49926-50228 [Escherichia coli O145:H28 str. RM13514]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|