Detailed information of TA system TA045184

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsRA (relBE)/MqsR-MqsA
• Location: 3819839..3820532
• Replicon: chromosome
• Accession: NZ_CP006027
• Organism: Escherichia coli O145:H28 str. RM13514

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: S1EZG2
• Locus tag: ECRM13514_RS19570
• Protein ID: WP_000415584.1
• Coordinates: 3820236..3820532 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: mqsA
• Uniprot ID: S1EBV2
• Locus tag: ECRM13514_RS19565
• Protein ID: WP_000650107.1
• Coordinates: 3819839..3820234 (-)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECRM13514_RS19555	3815703..3817961	-	2259	WP_001281878.1	DNA topoisomerase IV subunit A	-
ECRM13514_RS19560	3818099..3819706	-	1608	WP_001400746.1	ABC transporter substrate-binding protein	-
ECRM13514_RS19565	3819839..3820234	-	396	WP_000650107.1	type II toxin-antitoxin system antitoxin MqsA	Antitoxin
ECRM13514_RS19570	3820236..3820532	-	297	WP_000415584.1	type II toxin-antitoxin system toxin MqsR	Toxin
ECRM13514_RS19575	3820737..3821219	-	483	WP_000183497.1	GyrI-like domain-containing protein	-
ECRM13514_RS19580	3821272..3821664	-	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
ECRM13514_RS19585	3821816..3822475	+	660	WP_001221502.1	two-component system response regulator QseB	-
ECRM13514_RS19590	3822472..3823821	+	1350	WP_000673396.1	two-component system sensor histidine kinase QseC	-
ECRM13514_RS19595	3823903..3824199	-	297	WP_000912118.1	DUF2645 family protein	-
ECRM13514_RS19600	3824557..3825138	+	582	WP_000065430.1	NADPH:quinone oxidoreductase MdaB	-
ECRM13514_RS19605	3825169..3825483	+	315	WP_000958598.1	antibiotic biosynthesis monooxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (5-97)

Antitoxin

• Zinc finger, MqsA-type (3-47)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11231.96 Da
• Isoelectric Point: 8.9070

>T45184 WP_000415584.1 NZ_CP006027:c3820532-3820236 [Escherichia coli O145:H28 str. RM13514]
MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCNVIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQV
YLKITVIHDVLIVSFKEK

Nucleotide

• Length: 297 bp

>T45184 NZ_CP006027:c3820532-3820236 [Escherichia coli O145:H28 str. RM13514]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCACTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGACTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14703.10 Da
• Isoelectric Point: 9.2136

>AT45184 WP_000650107.1 NZ_CP006027:c3820234-3819839 [Escherichia coli O145:H28 str. RM13514]
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR

Nucleotide

• Length: 396 bp

>AT45184 NZ_CP006027:c3820234-3819839 [Escherichia coli O145:H28 str. RM13514]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACACAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Structures

Toxin

• PDB: 3HI2

Antitoxin

• PDB: 3HI2
• PDB: 3GA8
• PDB: 3O9X
• PDB: 2KZ8
• PDB: 3FMY
• PDB: 3GN5
• AlphaFold DB: A0A7U9LU65