Detailed information of TA system TA045180

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 2617023..2617525
• Replicon: chromosome
• Accession: NZ_CP006027
• Organism: Escherichia coli O145:H28 str. RM13514

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: U9YDH0
• Locus tag: ECRM13514_RS13715
• Protein ID: WP_000767829.1
• Coordinates: 2617023..2617277 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: S1Q063
• Locus tag: ECRM13514_RS13720
• Protein ID: WP_001259255.1
• Coordinates: 2617274..2617525 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECRM13514_RS13690	2612038..2612265	-	228	WP_000985273.1	sulfurtransferase TusA family protein	-
ECRM13514_RS13695	2612279..2613337	-	1059	WP_000492332.1	transport protein YeeE	-
ECRM13514_RS13700	2613516..2614874	-	1359	WP_000019197.1	putrescine/proton symporter PlaP	-
ECRM13514_RS33715	2614864..2614926	-	63	WP_010723108.1	membrane protein YoeI	-
ECRM13514_RS13705	2615141..2616070	-	930	WP_000803351.1	LysR family transcriptional regulator	-
ECRM13514_RS13710	2616116..2616940	-	825	WP_000754755.1	SDR family oxidoreductase	-
ECRM13514_RS13715	2617023..2617277	-	255	WP_000767829.1	type II toxin-antitoxin system mRNA interferase toxin YoeB	Toxin
ECRM13514_RS13720	2617274..2617525	-	252	WP_001259255.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
ECRM13514_RS31145	2617809..2617859	+	51	WP_001364200.1	his operon leader peptide	-
ECRM13514_RS13725	2618005..2618904	+	900	WP_000131782.1	ATP phosphoribosyltransferase	-
ECRM13514_RS13730	2618910..2620214	+	1305	WP_001400693.1	histidinol dehydrogenase	-
ECRM13514_RS13735	2620211..2621281	+	1071	WP_001099213.1	histidinol-phosphate transaminase	-
ECRM13514_RS13740	2621281..2622348	+	1068	WP_000080083.1	bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	ugd / gnd / cap8E	2551441..2634060	82619

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10183.58 Da
• Isoelectric Point: 8.0353

>T45180 WP_000767829.1 NZ_CP006027:c2617277-2617023 [Escherichia coli O145:H28 str. RM13514]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Nucleotide

• Length: 255 bp

>T45180 NZ_CP006027:c2617277-2617023 [Escherichia coli O145:H28 str. RM13514]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9307.58 Da
• Isoelectric Point: 4.7954

>AT45180 WP_001259255.1 NZ_CP006027:c2617525-2617274 [Escherichia coli O145:H28 str. RM13514]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Nucleotide

• Length: 252 bp

>AT45180 NZ_CP006027:c2617525-2617274 [Escherichia coli O145:H28 str. RM13514]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• PDB: 6NY6
• PDB: 2A6S
• PDB: 6OXA
• PDB: 2A6R
• PDB: 4V8X
• PDB: 6OTR
• PDB: 6OXI
• PDB: 2A6Q

Antitoxin

• PDB: 2A6Q
• AlphaFold DB: A0A7U9LNR0