Detailed information of TA system TA045167

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 1740149..1740520
• Replicon: chromosome
• Accession: NZ_CP006027
• Organism: Escherichia coli O145:H28 str. RM13514

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: A0A839BBC2
• Locus tag: ECRM13514_RS08875
• Protein ID: WP_021500490.1
• Coordinates: 1740326..1740520 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	1740149..1740327 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECRM13514_RS08850	1736103..1737476	+	1374	WP_000123735.1	ATP-dependent RNA helicase DbpA	-
ECRM13514_RS08855	1737605..1738540	-	936	WP_001157377.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
ECRM13514_RS30690	1738593..1739828	-	1236	WP_000040838.1	site-specific integrase	-
ECRM13514_RS08865	1739830..1740045	-	216	WP_000079604.1	excisionase XisR	-
ECRM13514_RS08870	1740145..1740333	-	189	WP_001298826.1	DUF1187 family protein	-
-	1740149..1740327	+	179	NuclAT_0	-	Antitoxin
-	1740149..1740327	+	179	NuclAT_0	-	Antitoxin
-	1740149..1740327	+	179	NuclAT_0	-	Antitoxin
-	1740149..1740327	+	179	NuclAT_0	-	Antitoxin
ECRM13514_RS08875	1740326..1740520	-	195	WP_021500490.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
ECRM13514_RS08880	1740584..1741636	-	1053	WP_001004423.1	recombinase RecT	-
ECRM13514_RS08885	1741648..1744773	-	3126	WP_025404324.1	exodeoxyribonuclease VIII	-
ECRM13514_RS08890	1744875..1745150	-	276	WP_001519830.1	phage protein	-
ECRM13514_RS08895	1745225..1745401	-	177	WP_000245528.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1731333..1806218	74885

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7046.98 Da
• Isoelectric Point: 9.2886

>T45167 WP_021500490.1 NZ_CP006027:c1740520-1740326 [Escherichia coli O145:H28 str. RM13514]
MRYEKVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T45167 NZ_CP006027:c1740520-1740326 [Escherichia coli O145:H28 str. RM13514]
ATGCGGTATGAAAAAGTCAAACCATGCCCTTTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
CGCGAAGTGTAACGGATGCGAATCCCGAACCGGCTATGGTGGAAGTGAAAAAGAAGCACTCGAACGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT45167 NZ_CP006027:1740149-1740327 [Escherichia coli O145:H28 str. RM13514]
GAGGACTGAAGTTGCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTGGTTAATTTTGTTTTTGAGTACCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A839BBC2

Antitoxin