Detailed information of TA system TA045137

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/-
• Location: 1345891..1346545
• Replicon: chromosome
• Accession: NZ_CP005960
• Organism: Pseudomonas mandelii JR-1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: OU5_RS05925
• Protein ID: WP_010458844.1
• Coordinates: 1345891..1346241 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A024E6Q4
• Locus tag: OU5_RS05930
• Protein ID: WP_010458842.1
• Coordinates: 1346231..1346545 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OU5_RS05915	1342599..1344131	+	1533	WP_010458848.1	NADH-quinone oxidoreductase subunit M	-
OU5_RS05920	1344139..1345602	+	1464	WP_010458846.1	NADH-quinone oxidoreductase subunit NuoN	-
OU5_RS05925	1345891..1346241	+	351	WP_010458844.1	toxin	Toxin
OU5_RS05930	1346231..1346545	+	315	WP_010458842.1	XRE family transcriptional regulator	Antitoxin
OU5_RS05935	1346720..1348045	+	1326	WP_010458840.1	OprD family porin	-
OU5_RS05940	1348214..1348546	-	333	WP_010458838.1	DUF2790 domain-containing protein	-
OU5_RS05945	1348626..1348826	-	201	WP_010458836.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13976.75 Da
• Isoelectric Point: 7.1657

>T45137 WP_010458844.1 NZ_CP005960:1345891-1346241 [Pseudomonas mandelii JR-1]
MDALFIELRMFQKHRDEYLDDDLFRSFQLELLKNPEAGDLIEGTGGLRKIRFCDQRRGKGKRGGLRVIYYWWSGFDQFWL
FTVYSKNEQDDLSPSQKNIFRQALDREINTRSHYET

Nucleotide

• Length: 351 bp

>T45137 NZ_CP005960:1345891-1346241 [Pseudomonas mandelii JR-1]
ATGGATGCTCTTTTCATCGAATTACGTATGTTCCAGAAACATCGAGACGAGTACCTGGATGACGATCTGTTTCGCAGCTT
TCAACTGGAGTTGCTGAAGAATCCAGAAGCGGGCGATCTCATTGAGGGCACCGGTGGGCTGCGCAAAATCCGCTTCTGCG
ATCAACGACGAGGCAAGGGCAAGCGCGGCGGATTGCGGGTGATTTATTACTGGTGGTCAGGCTTCGATCAATTCTGGCTG
TTCACCGTGTACAGCAAAAACGAGCAAGACGACCTGTCGCCCTCCCAGAAAAATATTTTCAGACAAGCGCTAGATAGAGA
AATCAACACGAGATCCCACTATGAAACGTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11972.96 Da
• Isoelectric Point: 9.9896

>AT45137 WP_010458842.1 NZ_CP005960:1346231-1346545 [Pseudomonas mandelii JR-1]
MKRDIFSELMDGLEALAEERQGKITLRTHKVQLPKLMPITAEEVVAIRQQLNLSRPVFAMYLRTNTRTLENWEQGRAIPN
AQATTLIRLVERFPQTIEQLAALT

Nucleotide

• Length: 315 bp

>AT45137 NZ_CP005960:1346231-1346545 [Pseudomonas mandelii JR-1]
ATGAAACGTGACATTTTTTCCGAACTGATGGATGGCCTCGAAGCGCTGGCCGAAGAGCGCCAAGGCAAGATCACTCTGCG
CACCCACAAGGTCCAGCTGCCAAAACTGATGCCCATCACGGCTGAGGAAGTGGTTGCCATCCGCCAACAGCTCAACCTCT
CCCGGCCAGTGTTCGCCATGTATCTGCGCACCAACACCCGAACCCTTGAGAACTGGGAACAGGGACGGGCAATACCCAAT
GCCCAAGCCACGACCCTGATACGTCTGGTTGAGCGCTTTCCGCAAACGATCGAACAGCTCGCAGCCCTGACCTGA

Structures

Antitoxin

• AlphaFold DB: A0A024E6Q4