Detailed information of TA system TA045132

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 131793..132218
• Replicon: plasmid p1ColV5155
• Accession: NZ_CP005931
• Organism: Escherichia coli APEC IMT5155

Toxin (Protein)

• Gene name: hok
• Uniprot ID: A0A0D8W959
• Locus tag: L282_RS25505
• Protein ID: WP_001332444.1
• Coordinates: 132057..132218 (+)
• Length: 54 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	131793..132004 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L282_RS25475	127767..128264	+	498	WP_050575774.1	single-stranded DNA-binding protein	-
L282_RS25480	128327..128560	+	234	WP_001209608.1	DUF905 domain-containing protein	-
L282_RS29265	128619..130577	+	1959	Protein_122	ParB/RepB/Spo0J family partition protein	-
L282_RS25490	130632..131066	+	435	WP_000845935.1	conjugation system SOS inhibitor PsiB	-
L282_RS25495	131063..131782	+	720	WP_001276275.1	plasmid SOS inhibition protein A	-
-	131793..132004	+	212	NuclAT_0	-	Antitoxin
-	131793..132004	+	212	NuclAT_0	-	Antitoxin
-	131793..132004	+	212	NuclAT_0	-	Antitoxin
-	131793..132004	+	212	NuclAT_0	-	Antitoxin
L282_RS25505	132057..132218	+	162	WP_001332444.1	type I toxin-antitoxin system Hok family toxin	Toxin
L282_RS29270	132456..133067	-	612	Protein_126	hypothetical protein	-
L282_RS25520	133246..134067	+	822	WP_001234485.1	DUF945 domain-containing protein	-
L282_RS25525	134363..134953	-	591	WP_000252676.1	transglycosylase SLT domain-containing protein	-
L282_RS25530	135287..135670	+	384	WP_000124981.1	relaxosome protein TraM	-
L282_RS25535	135860..136546	+	687	WP_000332490.1	PAS domain-containing protein	-
L282_RS25540	136640..136867	+	228	WP_001442099.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	iroN / iroE / iroD / iroC / iroB / iucA / iucB / iucC / iucD / iutA / vat	1..194170	194170
	-	flank	IS/Tn	-	-	126417..126920	503

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-48)

Sequences

Toxin

Protein

• Length: 54 a.a.
• Molecular weight: 5976.19 Da
• Isoelectric Point: 8.5101

>T45132 WP_001332444.1 NZ_CP005931:132057-132218 [Escherichia coli APEC IMT5155]
MKLPGNALIWCVLIVCCTLLIFTLLTRNRLCEVRLKDGYREVTASLAYESSGK

Nucleotide

• Length: 162 bp

>T45132 NZ_CP005931:132057-132218 [Escherichia coli APEC IMT5155]
ATGAAACTACCGGGAAACGCCCTTATCTGGTGCGTATTAATCGTGTGCTGCACGTTGTTAATATTCACACTCCTGACCCG
GAATCGTCTGTGCGAAGTCCGGCTGAAGGACGGATACAGGGAGGTTACGGCAAGTTTGGCTTACGAATCCAGCGGTAAGT
AG

Antitoxin

Nucleotide

• Length: 212 bp

>AT45132 NZ_CP005931:131793-132004 [Escherichia coli APEC IMT5155]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTCTTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
AGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGGC
AGAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGCCCCCTGTATGTCT

Structures

Toxin

• AlphaFold DB: A0A0D8W959

Antitoxin