Detailed information of TA system TA045127

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 34719..34973
• Replicon: plasmid p1ColV5155
• Accession: NZ_CP005931
• Organism: Escherichia coli APEC IMT5155

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: L282_RS25000
• Protein ID: WP_001351576.1
• Coordinates: 34719..34925 (-)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	34912..34973 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L282_RS24955	30262..30609	+	348	Protein_29	IS1-like element IS1A family transposase	-
L282_RS24965	31049..31336	-	288	WP_000222760.1	type II toxin-antitoxin system RelE/ParE family toxin	-
L282_RS24970	31333..31584	-	252	WP_001132900.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
L282_RS24980	32547..33404	-	858	WP_000774297.1	incFII family plasmid replication initiator RepA	-
L282_RS24985	33397..33879	-	483	WP_001273588.1	hypothetical protein	-
L282_RS24990	33872..33946	-	75	WP_001442103.1	RepA leader peptide Tap	-
L282_RS24995	34178..34435	-	258	WP_000083845.1	replication regulatory protein RepA	-
L282_RS25000	34719..34925	-	207	WP_001351576.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	34912..34973	+	62	NuclAT_1	-	Antitoxin
-	34912..34973	+	62	NuclAT_1	-	Antitoxin
-	34912..34973	+	62	NuclAT_1	-	Antitoxin
-	34912..34973	+	62	NuclAT_1	-	Antitoxin
L282_RS29535	35229..35303	-	75	Protein_37	endonuclease	-
L282_RS25005	35549..35761	-	213	WP_040111969.1	ANR family transcriptional regulator	-
L282_RS25010	35897..36457	-	561	WP_040111971.1	fertility inhibition protein FinO	-
L282_RS25015	36560..37420	-	861	WP_000704513.1	alpha/beta hydrolase	-
L282_RS25020	37479..38225	-	747	WP_021512286.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	iroN / iroE / iroD / iroC / iroB / iucA / iucB / iucC / iucD / iutA / vat	1..194170	194170

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7826.31 Da
• Isoelectric Point: 8.8807

>T45127 WP_001351576.1 NZ_CP005931:c34925-34719 [Escherichia coli APEC IMT5155]
MKYLNTTDCSLFFAERSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T45127 NZ_CP005931:c34925-34719 [Escherichia coli APEC IMT5155]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCTTTGCTGAGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAGCTGAATATTCACAGAG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT45127 NZ_CP005931:34912-34973 [Escherichia coli APEC IMT5155]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin