Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 4627562..4628124 Replicon chromosome
Accession NZ_CP005930
Organism Escherichia coli APEC IMT5155

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag L282_RS23215 Protein ID WP_000605675.1
Coordinates 4627562..4627840 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag L282_RS23220 Protein ID WP_000781370.1
Coordinates 4627840..4628124 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L282_RS23190 4623149..4623580 - 432 WP_000152310.1 peroxiredoxin OsmC -
L282_RS23195 4623925..4624140 + 216 WP_000495766.1 biofilm-dependent modulation protein -
L282_RS23200 4624242..4624379 + 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
L282_RS23205 4624535..4626232 + 1698 WP_000433462.1 malate dehydrogenase -
L282_RS23210 4626366..4627376 + 1011 WP_000642412.1 alcohol dehydrogenase AdhP -
L282_RS23215 4627562..4627840 + 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
L282_RS23220 4627840..4628124 + 285 WP_000781370.1 HigA family addiction module antidote protein Antitoxin
L282_RS23225 4628353..4629006 - 654 WP_000045647.1 formate dehydrogenase-N subunit gamma -
L282_RS23230 4628999..4629883 - 885 WP_001240584.1 formate dehydrogenase N subunit beta -
L282_RS23235 4629896..4632943 - 3048 WP_011478186.1 formate dehydrogenase-N subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T45125 WP_000605675.1 NZ_CP005930:4627562-4627840 [Escherichia coli APEC IMT5155]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T45125 NZ_CP005930:4627562-4627840 [Escherichia coli APEC IMT5155]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCAGCATGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTAC
ATCCGTTAAGCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGAATCACATTTGAATTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT45125 WP_000781370.1 NZ_CP005930:4627840-4628124 [Escherichia coli APEC IMT5155]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT45125 NZ_CP005930:4627840-4628124 [Escherichia coli APEC IMT5155]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TAAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAATGCCTGGAGTCTGGCAGAGGCGGAA
AAAACGGTGGATGTGTCGCGACTGCGCCGTCTGGTTACGCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References