Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 3446563..3447242 Replicon chromosome
Accession NZ_CP005930
Organism Escherichia coli APEC IMT5155

Toxin (Protein)


Gene name higB Uniprot ID V0VBP6
Locus tag L282_RS17000 Protein ID WP_000057524.1
Coordinates 3446563..3446865 (+) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag L282_RS17005 Protein ID WP_000806442.1
Coordinates 3446901..3447242 (+) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L282_RS16975 3441940..3443591 + 1652 Protein_3198 bifunctional UDP-sugar hydrolase/5'-nucleotidase -
L282_RS16980 3443629..3444132 - 504 WP_000667000.1 hypothetical protein -
L282_RS16985 3444129..3444929 - 801 WP_000439798.1 hypothetical protein -
L282_RS16990 3444953..3445432 - 480 WP_000186633.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
L282_RS16995 3445636..3446430 - 795 WP_000365177.1 TraB/GumN family protein -
L282_RS17000 3446563..3446865 + 303 WP_000057524.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
L282_RS17005 3446901..3447242 + 342 WP_000806442.1 HigA family addiction module antidote protein Antitoxin
L282_RS17010 3447300..3449804 - 2505 WP_000083945.1 copper-exporting P-type ATPase CopA -
L282_RS17015 3450066..3450998 + 933 WP_000883052.1 glutaminase A -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3443629..3455843 12214


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11839.47 Da        Isoelectric Point: 10.2237

>T45115 WP_000057524.1 NZ_CP005930:3446563-3446865 [Escherichia coli APEC IMT5155]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHWDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

>T45115 NZ_CP005930:3446563-3446865 [Escherichia coli APEC IMT5155]
ATGGCTCAAAAGAAAAATATAAGGAGTTTTCGTGATGCCTGGTTGGCTGATTTTTTTGTCCATTCAACGCCACATCGAAA
GATCCCTGCTGAAATCCATACCACGTTATCACGAAAGTTGGACATCATTAATGCTGCCACTTCTCATTGGGATTTACGCT
CCCCTCCGGGGAACCGATACGAAGAGTTATCCGGGAAATTACAGGAATACTCCTCCATTAGAGTGAATAAACAGTACCGA
CTGATTTTTAAATGGGTTAATGGCAAGGCCGAAGAATTGTTTTTAGACCCACACAACTACTAA

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT45115 WP_000806442.1 NZ_CP005930:3446901-3447242 [Escherichia coli APEC IMT5155]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp

>AT45115 NZ_CP005930:3446901-3447242 [Escherichia coli APEC IMT5155]
ATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAAT
CAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACCACTG
AGATGGCATTCCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCAGTTGATCTTTGG
GAAGTTGAAAACAACATGCGTACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGA
GCGTGCAAAAAAGGTCGCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0VBP6

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References