Detailed information of TA system TA045113

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3200093..3200787
• Replicon: chromosome
• Accession: NZ_CP005930
• Organism: Escherichia coli APEC IMT5155

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1PJQ2
• Locus tag: L282_RS15790
• Protein ID: WP_001263500.1
• Coordinates: 3200389..3200787 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: L282_RS15785
• Protein ID: WP_000554758.1
• Coordinates: 3200093..3200386 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L282_RS15765	3195629..3196126	+	498	WP_039267782.1	REP-associated tyrosine transposase RayT	-
L282_RS29370	3196209..3196367	-	159	WP_014639450.1	hypothetical protein	-
L282_RS15770	3196446..3198158	-	1713	Protein_2961	flagellar biosynthesis protein FlhA	-
L282_RS15775	3198130..3198915	+	786	WP_000207544.1	putative lateral flagellar export/assembly protein LafU	-
L282_RS15780	3198986..3200041	+	1056	WP_001226168.1	DNA polymerase IV	-
L282_RS15785	3200093..3200386	+	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
L282_RS15790	3200389..3200787	+	399	WP_001263500.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
L282_RS15795	3200797..3201249	+	453	WP_001059895.1	GNAT family N-acetyltransferase	-
L282_RS15800	3201439..3202578	+	1140	WP_039267783.1	RNA ligase RtcB family protein	-
L282_RS15805	3202575..3203189	+	615	WP_000602123.1	peptide chain release factor H	-
L282_RS15810	3203246..3204703	-	1458	WP_001292999.1	cytosol nonspecific dipeptidase	-
L282_RS15815	3204964..3205422	+	459	WP_001291988.1	xanthine phosphoribosyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	gmhA/lpcA	3180568..3201249	20681

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15413.85 Da
• Isoelectric Point: 8.9511

>T45113 WP_001263500.1 NZ_CP005930:3200389-3200787 [Escherichia coli APEC IMT5155]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T45113 NZ_CP005930:3200389-3200787 [Escherichia coli APEC IMT5155]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGAAGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGTGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGCAGCGCATTTGGTATATTGTCAGGGCGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT45113 WP_000554758.1 NZ_CP005930:3200093-3200386 [Escherichia coli APEC IMT5155]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT45113 NZ_CP005930:3200093-3200386 [Escherichia coli APEC IMT5155]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
TGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTTGAAGCATTAATGGACATGCTTG
CAGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCGAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1PJQ2

Antitoxin

• AlphaFold DB: S1QAE3