Detailed information of TA system TA045107

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 2221741..2222343
• Replicon: chromosome
• Accession: NZ_CP005930
• Organism: Escherichia coli APEC IMT5155

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: L282_RS11055
• Protein ID: WP_000897302.1
• Coordinates: 2221741..2222052 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: L282_RS11060
• Protein ID: WP_000356397.1
• Coordinates: 2222053..2222343 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L282_RS11030	2217655..2218254	+	600	WP_001314324.1	glucose-1-phosphatase	-
L282_RS11035	2218248..2219120	+	873	WP_000920762.1	virulence factor BrkB family protein	-
L282_RS11040	2219117..2219554	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
L282_RS11045	2219599..2220540	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
L282_RS11050	2220604..2221512	-	909	WP_001331553.1	alpha/beta hydrolase	-
L282_RS11055	2221741..2222052	+	312	WP_000897302.1	hypothetical protein	Toxin
L282_RS11060	2222053..2222343	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
L282_RS11070	2222702..2222980	+	279	WP_001296612.1	hypothetical protein	-
L282_RS11075	2223376..2223594	+	219	WP_001314326.1	ribbon-helix-helix domain-containing protein	-
L282_RS11080	2223810..2224739	-	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
L282_RS11085	2224736..2225371	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
L282_RS11090	2225368..2226270	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T45107 WP_000897302.1 NZ_CP005930:2221741-2222052 [Escherichia coli APEC IMT5155]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T45107 NZ_CP005930:2221741-2222052 [Escherichia coli APEC IMT5155]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT45107 WP_000356397.1 NZ_CP005930:2222053-2222343 [Escherichia coli APEC IMT5155]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT45107 NZ_CP005930:2222053-2222343 [Escherichia coli APEC IMT5155]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: S1P416