Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 121807..122309 Replicon chromosome
Accession NZ_CP005930
Organism Escherichia coli APEC IMT5155

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag L282_RS00700 Protein ID WP_000767829.1
Coordinates 121807..122061 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag L282_RS00705 Protein ID WP_001259255.1
Coordinates 122058..122309 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L282_RS00675 116822..117049 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
L282_RS00680 117063..118121 - 1059 WP_023568741.1 transport protein YeeE -
L282_RS00685 118300..119658 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
L282_RS28735 119648..119710 - 63 WP_010723108.1 membrane protein YoeI -
L282_RS00690 119925..120854 - 930 WP_000803351.1 LysR family transcriptional regulator -
L282_RS00695 120900..121724 - 825 WP_000754737.1 SDR family oxidoreductase -
L282_RS00700 121807..122061 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
L282_RS00705 122058..122309 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
L282_RS26195 122592..122642 + 51 WP_071593552.1 his operon leader peptide -
L282_RS00710 122788..123687 + 900 WP_000131789.1 ATP phosphoribosyltransferase -
L282_RS00715 123693..124997 + 1305 WP_039267630.1 histidinol dehydrogenase -
L282_RS00720 124994..126064 + 1071 WP_000108943.1 histidinol-phosphate transaminase -
L282_RS00725 126064..127131 + 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T45104 WP_000767829.1 NZ_CP005930:c122061-121807 [Escherichia coli APEC IMT5155]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T45104 NZ_CP005930:c122061-121807 [Escherichia coli APEC IMT5155]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT45104 WP_001259255.1 NZ_CP005930:c122309-122058 [Escherichia coli APEC IMT5155]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT45104 NZ_CP005930:c122309-122058 [Escherichia coli APEC IMT5155]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References