Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 746680..747330 Replicon chromosome
Accession NZ_CP004887
Organism Klebsiella michiganensis HKOPL1

Toxin (Protein)


Gene name higB Uniprot ID A0A0H3H3K0
Locus tag J415_RS03465 Protein ID WP_014227314.1
Coordinates 746680..747021 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A0H3H3Q5
Locus tag J415_RS03470 Protein ID WP_014227313.1
Coordinates 747031..747330 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J415_RS03450 742672..743991 + 1320 WP_014227316.1 MFS transporter -
J415_RS03455 744137..745528 + 1392 WP_014227315.1 hexose-6-phosphate:phosphate antiporter -
J415_RS03460 745977..746429 + 453 WP_009653860.1 DUF1198 domain-containing protein -
J415_RS03465 746680..747021 + 342 WP_014227314.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
J415_RS03470 747031..747330 + 300 WP_014227313.1 helix-turn-helix transcriptional regulator Antitoxin
J415_RS03475 747449..748630 + 1182 WP_014227312.1 purine ribonucleoside efflux pump NepI -
J415_RS03480 748663..749472 - 810 WP_038422921.1 phosphonoacetaldehyde hydrolase -
J415_RS03485 749482..750585 - 1104 WP_014227310.1 2-aminoethylphosphonate--pyruvate transaminase -
J415_RS03490 750726..751445 + 720 WP_014227309.1 phosphonate utilization transcriptional regulator PhnR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-107)

Antitoxin

Cro/C1-type helix-turn-helix domain

(31-84)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13110.00 Da        Isoelectric Point: 5.7545

>T45047 WP_014227314.1 NZ_CP004887:746680-747021 [Klebsiella michiganensis HKOPL1]
MWDVETTEVFDKWFEAQTEALKEDMLAAMVILSEYGPRLGRPFADTVNDSAFSNMKELRIQHQGRPIRAFFVFDPSRCGI
VLCAGDKTGLNEKRFYKDMIKLADAEYRKHLNQ

Download         Length: 342 bp

>T45047 NZ_CP004887:746680-747021 [Klebsiella michiganensis HKOPL1]
ATGTGGGATGTTGAAACAACGGAGGTTTTTGACAAGTGGTTTGAGGCGCAGACCGAAGCGCTCAAAGAGGATATGCTCGC
CGCAATGGTGATCTTATCCGAGTATGGCCCCCGGTTGGGAAGGCCGTTTGCGGATACAGTAAACGATTCTGCCTTTTCGA
ATATGAAAGAGCTGCGCATTCAGCATCAAGGTAGACCCATCCGAGCTTTCTTTGTATTTGACCCTTCTCGTTGTGGCATC
GTGTTATGCGCCGGAGATAAAACAGGGTTAAATGAAAAGAGATTCTATAAAGATATGATCAAGCTTGCAGATGCTGAGTA
CCGTAAGCACCTTAATCAGTAA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10966.87 Da        Isoelectric Point: 9.7256

>AT45047 WP_014227313.1 NZ_CP004887:747031-747330 [Klebsiella michiganensis HKOPL1]
MATLKELMAKQSPESQARIAEKVEEMRQAIALSMLREELNMSQAELAKAMGVKQPTIAKMEQADNDPRLSTLKRYVAALG
GELSIDVKLPTGKRIAFHL

Download         Length: 300 bp

>AT45047 NZ_CP004887:747031-747330 [Klebsiella michiganensis HKOPL1]
ATGGCGACCCTTAAAGAATTAATGGCCAAACAGAGCCCGGAAAGCCAGGCGCGTATTGCTGAAAAAGTCGAGGAGATGCG
CCAGGCTATCGCTTTAAGTATGTTAAGGGAAGAACTCAATATGTCTCAGGCAGAGCTGGCTAAGGCAATGGGCGTTAAAC
AACCCACCATAGCAAAAATGGAACAGGCTGATAACGACCCGCGTTTGTCAACGCTGAAGCGCTATGTTGCAGCGCTCGGG
GGGGAACTCAGTATTGATGTTAAGCTGCCAACCGGTAAGCGAATCGCATTCCATCTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3H3K0

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3H3Q5

References