Detailed information of TA system TA045006

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 2597762..2598419
• Replicon: chromosome
• Accession: NZ_CP004377
• Organism: Burkholderia pseudomallei NAU35A-3

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A0F6L6G8
• Locus tag: BBU_RS11435
• Protein ID: WP_009910242.1
• Coordinates: 2597762..2598094 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BBU_RS33545
• Protein ID: WP_076861268.1
• Coordinates: 2598102..2598419 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BBU_RS11410	2592954..2593859	+	906	WP_038795669.1	AAA family ATPase	-
BBU_RS11415	2593856..2595232	+	1377	WP_038795670.1	type II secretory pathway protein	-
BBU_RS11420	2595246..2595479	-	234	WP_038738754.1	hypothetical protein	-
BBU_RS11425	2596006..2596785	+	780	WP_038795671.1	hypothetical protein	-
BBU_RS11430	2597133..2597720	+	588	WP_038795917.1	site-specific integrase	-
BBU_RS11435	2597762..2598094	+	333	WP_009910242.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BBU_RS33545	2598102..2598419	+	318	WP_076861268.1	helix-turn-helix domain-containing protein	Antitoxin
BBU_RS33550	2598536..2598925	+	390	WP_200885081.1	DUF3892 domain-containing protein	-
BBU_RS33555	2599271..2599495	-	225	WP_004195757.1	hypothetical protein	-
BBU_RS11445	2599712..2600029	+	318	WP_004266306.1	hypothetical protein	-
BBU_RS11450	2600534..2601178	+	645	WP_004195754.1	lytic polysaccharide monooxygenase	-
BBU_RS11455	2601361..2603022	+	1662	WP_038745308.1	FAD-binding oxidoreductase	-
BBU_RS11460	2603170..2603304	-	135	WP_004198237.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2585332..2600029	14697

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (19-104)

Antitoxin

• Cro/C1-type helix-turn-helix domain (43-97)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 12849.92 Da
• Isoelectric Point: 10.3619

>T45006 WP_009910242.1 NZ_CP004377:2597762-2598094 [Burkholderia pseudomallei NAU35A-3]
MKFDWTVVYYNERVKRDVFAFPAGILADYLRLLDLMQEFGADLRMPHSRAMGNGLFELRPKGREGIGRVFYCTHVGRQVV
VLHSFVKKTQETPQHELRIAQARLREVRNG

Nucleotide

• Length: 333 bp

>T45006 NZ_CP004377:2597762-2598094 [Burkholderia pseudomallei NAU35A-3]
ATGAAATTCGATTGGACAGTCGTCTACTACAACGAACGGGTGAAGCGCGACGTTTTCGCGTTCCCGGCTGGGATTCTCGC
GGACTACCTCCGGCTGTTGGATCTGATGCAGGAGTTCGGGGCGGACCTGCGGATGCCGCACTCCCGAGCAATGGGTAACG
GGTTGTTTGAGTTGCGTCCCAAGGGTAGGGAAGGTATCGGGCGGGTGTTCTATTGCACGCATGTGGGGAGGCAAGTTGTT
GTGCTTCACTCCTTCGTTAAGAAGACACAGGAAACGCCGCAACATGAATTGCGGATCGCCCAAGCACGTTTGAGAGAGGT
GCGTAATGGCTAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11263.81 Da
• Isoelectric Point: 10.2231

>AT45006 WP_076861268.1 NZ_CP004377:2598102-2598419 [Burkholderia pseudomallei NAU35A-3]
VKRVRAEGFNPVPHTADDTARLLAGRNVKAAYDALEDEYTALRAILSARREAGLTQAQIAERMGTTASAVSRLEASLSSE
KHSPSFATLRKYAAACGKKLVISFA

Nucleotide

• Length: 318 bp

>AT45006 NZ_CP004377:2598102-2598419 [Burkholderia pseudomallei NAU35A-3]
GTGAAGCGTGTTCGCGCTGAGGGATTCAATCCGGTTCCGCATACGGCGGACGACACGGCGCGTCTGCTTGCCGGTCGCAA
TGTCAAGGCGGCGTATGACGCTTTGGAGGATGAGTACACCGCATTGCGCGCTATTTTGTCTGCGCGGCGCGAGGCTGGTT
TGACGCAGGCACAGATTGCGGAGCGTATGGGGACGACTGCATCGGCGGTTTCGCGGCTTGAGGCGTCTCTGTCGAGTGAA
AAGCACTCACCGTCATTTGCGACGCTGCGGAAATATGCTGCTGCATGCGGGAAGAAGCTTGTAATTTCGTTTGCGTAA

Structures

Toxin

• AlphaFold DB: A0A0F6L6G8