Detailed information of TA system TA044999

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 317838..318379
• Replicon: plasmid pGX5
• Accession: NZ_CP004365
• Organism: Komagataeibacter xylinus E25

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: H845_RS19335
• Protein ID: WP_081749560.1
• Coordinates: 317838..318107 (-)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: W5YEV0
• Locus tag: H845_RS17895
• Protein ID: WP_025440213.1
• Coordinates: 318104..318379 (-)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H845_RS17870	312997..313455	+	459	WP_025440210.1	class I SAM-dependent methyltransferase	-
H845_RS20015	313531..314286	-	756	WP_133251155.1	hypothetical protein	-
H845_RS17875	314534..315313	-	780	WP_025440211.1	aromatic-ring-hydroxylating dioxygenase	-
H845_RS17880	315303..316640	-	1338	WP_025440212.1	hypothetical protein	-
H845_RS19330	317000..317272	-	273	Protein_324	transposase	-
H845_RS20310	317278..317825	-	548	Protein_325	hypothetical protein	-
H845_RS19335	317838..318107	-	270	WP_081749560.1	Txe/YoeB family addiction module toxin	Toxin
H845_RS17895	318104..318379	-	276	WP_025440213.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
H845_RS17900	318698..320266	-	1569	WP_025437224.1	IS66 family transposase	-
H845_RS17905	320332..320679	-	348	WP_019092242.1	IS66 family insertion sequence element accessory protein TnpB	-
H845_RS17910	320676..321059	-	384	WP_081749485.1	transposase	-
H845_RS17915	321489..322319	-	831	WP_011253091.1	IS5 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..336138	336138

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-89)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-75)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10486.83 Da
• Isoelectric Point: 8.1012

>T44999 WP_081749560.1 NZ_CP004365:c318107-317838 [Komagataeibacter xylinus E25]
VKVIFHEDGWEDYLSWFQSDHAVLGKVNALIEDVRRHPFQGIGKPEPLKGQLSGWWSRRITGEHRLVYRVQGRAGEDQRI
EVAQCRFHY

Nucleotide

• Length: 270 bp

>T44999 NZ_CP004365:c318107-317838 [Komagataeibacter xylinus E25]
GTGAAGGTCATTTTTCATGAAGATGGCTGGGAGGATTATCTTTCCTGGTTCCAGTCAGATCATGCTGTTCTTGGGAAGGT
TAATGCCCTGATCGAGGATGTAAGGCGGCATCCGTTTCAGGGGATAGGGAAGCCAGAACCACTGAAGGGGCAGCTTTCCG
GGTGGTGGTCCCGACGGATTACGGGGGAACATCGTCTGGTCTACCGGGTTCAGGGCCGCGCGGGAGAGGATCAGCGGATC
GAGGTTGCCCAGTGCCGGTTTCATTACTGA

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 10209.39 Da
• Isoelectric Point: 4.8498

>AT44999 WP_025440213.1 NZ_CP004365:c318379-318104 [Komagataeibacter xylinus E25]
MTCVSYTDLRQNLAHYLDEAVNSRAPIVVTRKTGKGSVVLMSEEEFSGWQETVHLLSSPRNAERLLRSIRDIEHGAGTER
DLLEPQDEPSA

Nucleotide

• Length: 276 bp

>AT44999 NZ_CP004365:c318379-318104 [Komagataeibacter xylinus E25]
ATGACCTGTGTTTCCTATACCGACCTGCGCCAGAATCTGGCACATTATCTGGACGAAGCCGTGAACAGCCGCGCGCCGAT
TGTCGTGACCCGCAAGACCGGGAAAGGCAGTGTCGTGCTGATGTCGGAAGAAGAATTCTCTGGCTGGCAGGAAACCGTGC
ATCTTCTGAGCAGCCCACGCAACGCGGAAAGGCTGTTGCGGAGCATTCGTGACATCGAGCATGGGGCGGGGACCGAGCGT
GATCTTCTGGAGCCGCAGGATGAACCGTCGGCGTGA

Structures

Antitoxin

• AlphaFold DB: W5YEV0