Detailed information of TA system TA044987

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 2844626..2845283
• Replicon: chromosome
• Accession: NZ_CP004117
• Organism: Burkholderia thailandensis E444

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: BTJ_RS12660
• Protein ID: WP_025369694.1
• Coordinates: 2844951..2845283 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BTJ_RS30575
• Protein ID: WP_025369693.1
• Coordinates: 2844626..2844958 (-)
• Length: 111 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BTJ_RS30570	2839818..2840366	-	549	WP_080556799.1	methylated-DNA--[protein]-cysteine S-methyltransferase	-
BTJ_RS12640	2840332..2840892	-	561	Protein_2503	LysR family transcriptional regulator	-
BTJ_RS12645	2840972..2841937	+	966	Protein_2504	IS256 family transposase	-
BTJ_RS12650	2842051..2843079	-	1029	WP_009910245.1	site-specific integrase	-
BTJ_RS33120	2843350..2844633	-	1284	WP_144314124.1	hypothetical protein	-
BTJ_RS30575	2844626..2844958	-	333	WP_025369693.1	helix-turn-helix domain-containing protein	Antitoxin
BTJ_RS12660	2844951..2845283	-	333	WP_025369694.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BTJ_RS12665	2845324..2845911	-	588	WP_025369695.1	tyrosine-type recombinase/integrase	-
BTJ_RS12670	2846259..2847038	-	780	WP_025369696.1	hypothetical protein	-
BTJ_RS12675	2847504..2848892	-	1389	WP_025369697.1	hypothetical protein	-
BTJ_RS12680	2848889..2849794	-	906	WP_009910232.1	hypothetical protein	-
BTJ_RS12685	2849791..2850072	-	282	WP_025369698.1	DUF2523 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2842051..2857632	15581
	-	flank	IS/Tn	-	-	2840948..2842138	1190

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (19-104)

Antitoxin

• Cro/C1-type helix-turn-helix domain (48-102)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 12878.87 Da
• Isoelectric Point: 10.1340

>T44987 WP_025369694.1 NZ_CP004117:c2845283-2844951 [Burkholderia thailandensis E444]
MKSDWTIVYYNERVKRDVFAFPAGILADYLRLLELMQEFGADLRMPHSRAMGDGLFELRPKGREGIGRVFYCTHVGRHVV
VLHSFVKKTQETPQNELRTARARLREVRND

Nucleotide

• Length: 333 bp

>T44987 NZ_CP004117:c2845283-2844951 [Burkholderia thailandensis E444]
ATGAAATCAGATTGGACGATCGTCTATTACAACGAGCGCGTCAAGCGCGACGTCTTCGCCTTCCCGGCCGGGATTCTGGC
TGACTATCTCCGCTTGCTGGAGCTGATGCAGGAGTTCGGGGCGGATTTGCGTATGCCGCACTCCCGGGCAATGGGGGACG
GGTTGTTCGAGTTGCGCCCAAAAGGGCGCGAAGGTATCGGGCGAGTGTTCTACTGCACGCATGTTGGGCGGCACGTGGTC
GTGCTGCACTCCTTTGTCAAGAAGACGCAGGAGACCCCGCAAAATGAATTGCGGACAGCCCGAGCACGATTGAGAGAGGT
ACGAAATGACTAA

Antitoxin

Protein

• Length: 111 a.a.
• Molecular weight: 11701.28 Da
• Isoelectric Point: 10.1772

>AT44987 WP_025369693.1 NZ_CP004117:c2844958-2844626 [Burkholderia thailandensis E444]
MTKVASKRARGEGFNPVPHTADDTARLLASSKVKAAYDALEDEYTALRAILAARQEAGLTQAQVAERMGTTASAVSRLEA
SLSSEKHSPSFSTLRKYAAACGKRLVISFA

Nucleotide

• Length: 333 bp

>AT44987 NZ_CP004117:c2844958-2844626 [Burkholderia thailandensis E444]
ATGACTAAGGTTGCATCGAAGCGCGCTCGCGGCGAGGGATTCAATCCGGTTCCTCATACAGCAGACGACACGGCTCGTCT
GCTTGCAAGTAGCAAGGTCAAGGCGGCCTATGACGCGCTGGAAGATGAATACACGGCGTTGCGCGCCATTTTGGCCGCGC
GGCAAGAGGCGGGATTGACGCAAGCGCAGGTCGCTGAGCGTATGGGAACAACGGCATCCGCTGTGTCGAGACTTGAGGCG
TCGCTGTCTAGCGAAAAGCATTCACCATCGTTCTCAACTTTGCGGAAGTACGCGGCTGCGTGTGGGAAGAGGCTCGTGAT
CTCATTTGCCTAA