Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3248169..3248896 Replicon chromosome
Accession NZ_CP004057
Organism Shigella flexneri Shi06HN006 isolate Human

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag SFYV_RS17725 Protein ID WP_000550189.1
Coordinates 3248582..3248896 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag SFYV_RS17720 Protein ID WP_000560264.1
Coordinates 3248169..3248585 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SFYV_RS17710 (3243529) 3243529..3245880 + 2352 WP_000695506.1 alpha-glucosidase -
SFYV_RS17715 (3246106) 3246106..3248124 + 2019 WP_000121487.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
SFYV_RS17720 (3248169) 3248169..3248585 - 417 WP_000560264.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
SFYV_RS17725 (3248582) 3248582..3248896 - 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
SFYV_RS17735 (3249181) 3249181..3250317 - 1137 WP_000018656.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
SFYV_RS17740 (3250402) 3250402..3250905 + 504 WP_005050988.1 M48 family metallopeptidase -
SFYV_RS17745 (3250982) 3250982..3251674 + 693 WP_000942538.1 vancomycin high temperature exclusion protein -
SFYV_RS17750 (3251753) 3251753..3252751 + 999 WP_005050984.1 Gfo/Idh/MocA family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T44953 WP_000550189.1 NZ_CP004057:c3248896-3248582 [Shigella flexneri Shi06HN006]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T44953 NZ_CP004057:c3248896-3248582 [Shigella flexneri Shi06HN006]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15064.53 Da        Isoelectric Point: 4.5822

>AT44953 WP_000560264.1 NZ_CP004057:c3248585-3248169 [Shigella flexneri Shi06HN006]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGRKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT44953 NZ_CP004057:c3248585-3248169 [Shigella flexneri Shi06HN006]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCCGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References