Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 397459..398077 Replicon chromosome
Accession NZ_CP004056
Organism Shigella flexneri 2003036 isolate Human

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag SFY_RS02060 Protein ID WP_001291435.1
Coordinates 397459..397677 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID Q0T7C3
Locus tag SFY_RS02065 Protein ID WP_000344797.1
Coordinates 397703..398077 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SFY_RS02035 393351..393662 - 312 WP_000409911.1 MGMT family protein -
SFY_RS02040 394041..394394 + 354 WP_000878140.1 DUF1428 domain-containing protein -
SFY_RS02045 394436..395986 - 1551 WP_005098218.1 cyclic-guanylate-specific phosphodiesterase PdeB -
SFY_RS02050 396150..396620 - 471 WP_000136192.1 YlaC family protein -
SFY_RS02055 396736..397287 - 552 Protein_372 maltose O-acetyltransferase -
SFY_RS02060 397459..397677 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
SFY_RS02065 397703..398077 - 375 WP_000344797.1 Hha toxicity modulator TomB Antitoxin
SFY_RS02070 398623..401772 - 3150 WP_001132484.1 multidrug efflux RND transporter permease subunit -
SFY_RS02075 401795..402988 - 1194 WP_011069267.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 391993..393321 1328


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T44915 WP_001291435.1 NZ_CP004056:c397677-397459 [Shigella flexneri 2003036]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T44915 NZ_CP004056:c397677-397459 [Shigella flexneri 2003036]
ATGTCCGAAAAACCTTTAACAAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14513.38 Da        Isoelectric Point: 4.9091

>AT44915 WP_000344797.1 NZ_CP004056:c398077-397703 [Shigella flexneri 2003036]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQALQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT44915 NZ_CP004056:c398077-397703 [Shigella flexneri 2003036]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGTTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGCTCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAACGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A4P7TPD8

References