Detailed information of TA system TA044914

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 360304..361141
• Replicon: chromosome
• Accession: NZ_CP004056
• Organism: Shigella flexneri 2003036 isolate Human

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: -
• Locus tag: SFY_RS01870
• Protein ID: WP_025748793.1
• Coordinates: 360304..360846 (-)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: A5H0K0
• Locus tag: SFY_RS01875
• Protein ID: WP_005053053.1
• Coordinates: 360830..361141 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SFY_RS01850	355472..357202	-	1731	WP_000645013.1	hypothetical protein	-
SFY_RS01855	357255..359408	-	2154	WP_005060811.1	SEL1-like repeat protein	-
SFY_RS01865	359559..360256	+	698	Protein_334	IS1 family transposase	-
SFY_RS01870	360304..360846	-	543	WP_025748793.1	GNAT family N-acetyltransferase	Toxin
SFY_RS01875	360830..361141	-	312	WP_005053053.1	DUF1778 domain-containing protein	Antitoxin
SFY_RS01880	361325..362215	-	891	WP_000971346.1	heme o synthase	-
SFY_RS01885	362227..362556	-	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
SFY_RS01890	362556..363170	-	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
SFY_RS01895	363160..365151	-	1992	Protein_340	cytochrome o ubiquinol oxidase subunit I	-
SFY_RS01900	365173..365670	-	498	Protein_341	COX aromatic rich motif-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	357255..367637	10382
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NZ_CP037923 Shigella flexneri 5a str. M90T 4168367..4168870 0.007	flank	IS/Tn	-	-	359753..360256	503

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (99-160)

Antitoxin

• Vibrio phage ICP1, Orf50 (15-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19774.95 Da
• Isoelectric Point: 8.6178

>T44914 WP_025748793.1 NZ_CP004056:c360846-360304 [Shigella flexneri 2003036]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLER
QRVSGVLQGSQPSEIGVVRLVMLGGARKYQKRGFGQDLLCDFFEHVKKIHPALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T44914 NZ_CP004056:c360846-360304 [Shigella flexneri 2003036]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATTGGCATCTGTACTTTTACGGCATATTCGCTGGAAAGA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGGAGCGCG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAAAAATTCACCCGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAACTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11394.07 Da
• Isoelectric Point: 10.3388

>AT44914 WP_005053053.1 NZ_CP004056:c361141-360830 [Shigella flexneri 2003036]
MPGKTAALYDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT44914 NZ_CP004056:c361141-360830 [Shigella flexneri 2003036]
ATGCCTGGAAAAACAGCCGCTCTATACGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Antitoxin

• AlphaFold DB: A5H0K0