Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 298897..299591 Replicon chromosome
Accession NZ_CP004056
Organism Shigella flexneri 2003036 isolate Human

Toxin (Protein)


Gene name yafO Uniprot ID Q0T7Q5
Locus tag SFY_RS01525 Protein ID WP_001263491.1
Coordinates 299193..299591 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID Q0T7Q4
Locus tag SFY_RS01520 Protein ID WP_000554759.1
Coordinates 298897..299190 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SFY_RS01500 294536..295033 + 498 WP_000006251.1 REP-associated tyrosine transposase RayT -
SFY_RS01505 295250..296989 - 1740 WP_005082921.1 flagellar biosynthesis protein FlhA -
SFY_RS01510 296934..297719 + 786 WP_000207560.1 putative lateral flagellar export/assembly protein LafU -
SFY_RS01515 297790..298845 + 1056 WP_001226172.1 DNA polymerase IV -
SFY_RS01520 298897..299190 + 294 WP_000554759.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
SFY_RS01525 299193..299591 + 399 WP_001263491.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
SFY_RS01530 299601..300053 + 453 WP_001059860.1 GNAT family N-acetyltransferase -
SFY_RS01535 300299..301342 + 1044 WP_005053205.1 RNA ligase RtcB family protein -
SFY_RS01540 301458..302018 + 561 Protein_278 peptide chain release factor H -
SFY_RS01545 302075..303532 - 1458 WP_001293030.1 cytosol nonspecific dipeptidase -
SFY_RS01550 303794..304252 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 297790..314926 17136


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15457.86 Da        Isoelectric Point: 8.0949

>T44913 WP_001263491.1 NZ_CP004056:299193-299591 [Shigella flexneri 2003036]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T44913 NZ_CP004056:299193-299591 [Shigella flexneri 2003036]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAGCCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGAAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11232.82 Da        Isoelectric Point: 7.0153

>AT44913 WP_000554759.1 NZ_CP004056:298897-299190 [Shigella flexneri 2003036]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
KQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT44913 NZ_CP004056:298897-299190 [Shigella flexneri 2003036]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
AGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTTCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
AAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TPK7

Antitoxin

Source ID Structure
AlphaFold DB A0A4P7TR46

References