Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 325195..325781 Replicon chromosome
Accession NZ_CP003999
Organism Klebsiella pneumoniae subsp. pneumoniae Kp13

Toxin (Protein)


Gene name doc Uniprot ID A0A9J6Y968
Locus tag KP13_RS02435 Protein ID WP_025367836.1
Coordinates 325413..325781 (+) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID W9B1V1
Locus tag KP13_RS02430 Protein ID WP_004174006.1
Coordinates 325195..325416 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KP13_RS02410 321352..322278 + 927 WP_002920807.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
KP13_RS02415 322275..323552 + 1278 WP_004188292.1 branched chain amino acid ABC transporter permease LivM -
KP13_RS02420 323549..324316 + 768 WP_002920803.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
KP13_RS02425 324318..325031 + 714 WP_004145133.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
KP13_RS02430 325195..325416 + 222 WP_004174006.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
KP13_RS02435 325413..325781 + 369 WP_025367836.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
KP13_RS02440 326054..327370 + 1317 WP_004174008.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
KP13_RS02445 327477..328364 + 888 WP_002920792.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
KP13_RS02450 328361..329206 + 846 WP_004145129.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
KP13_RS02455 329208..330278 + 1071 WP_004150074.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 322275..331015 8740


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(1-120)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-50)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13449.86 Da        Isoelectric Point: 7.2790

>T44875 WP_025367836.1 NZ_CP003999:325413-325781 [Klebsiella pneumoniae subsp. pneumoniae Kp13]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIECEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLLG

Download         Length: 369 bp

>T44875 NZ_CP003999:325413-325781 [Klebsiella pneumoniae subsp. pneumoniae Kp13]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATGTGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCTTGGATGA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8151.15 Da        Isoelectric Point: 4.7769

>AT44875 WP_004174006.1 NZ_CP003999:325195-325416 [Klebsiella pneumoniae subsp. pneumoniae Kp13]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Download         Length: 222 bp

>AT44875 NZ_CP003999:325195-325416 [Klebsiella pneumoniae subsp. pneumoniae Kp13]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A5E5YJY7

References