Detailed information of TA system TA044861

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ-RelB
• Location: 10864..11392
• Replicon: plasmid pZM1252603
• Accession: NZ_CP003712
• Organism: Zymomonas mobilis subsp. mobilis NRRL B-12526

Toxin (Protein)

• Gene name: yafQ
• Uniprot ID: A0A806D8N5
• Locus tag: A254_RS09240
• Protein ID: WP_012954670.1
• Coordinates: 11123..11392 (+)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: A0A806DA69
• Locus tag: A254_RS09235
• Protein ID: WP_012954669.1
• Coordinates: 10864..11121 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A254_RS09830	6150..6578	+	429	WP_012954715.1	helix-turn-helix domain-containing protein	-
A254_RS09210	6589..6870	+	282	WP_012954716.1	DUF3467 domain-containing protein	-
A254_RS09215	6957..7901	+	945	WP_012954717.1	hypothetical protein	-
A254_RS09220	8262..8951	+	690	WP_012954718.1	hypothetical protein	-
A254_RS09225	8984..10189	+	1206	WP_012954719.1	XRE family transcriptional regulator	-
A254_RS09230	10407..10709	-	303	Protein_14	helix-turn-helix domain-containing protein	-
A254_RS09235	10864..11121	+	258	WP_012954669.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
A254_RS09240	11123..11392	+	270	WP_012954670.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	Toxin
A254_RS09920	11645..11920	+	276	WP_023593779.1	hypothetical protein	-
A254_RS09250	12396..12758	+	363	WP_023593780.1	hypothetical protein	-
A254_RS09255	12769..13095	+	327	WP_012954673.1	hypothetical protein	-
A254_RS09675	13200..13526	+	327	WP_012954674.1	hypothetical protein	-
A254_RS09265	13631..13957	+	327	WP_012954675.1	hypothetical protein	-
A254_RS09270	14117..14389	+	273	WP_012954676.1	hypothetical protein	-
A254_RS09275	14469..14795	+	327	WP_025420259.1	hypothetical protein	-
A254_RS09280	14776..15768	-	993	WP_012954678.1	late control D family protein	-
A254_RS09680	15765..16310	-	546	WP_012954679.1	phage tail protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..37061	37061

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (7-88)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (5-80)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10489.31 Da
• Isoelectric Point: 10.0219

>T44861 WP_012954670.1 NZ_CP003712:11123-11392 [Zymomonas mobilis subsp. mobilis NRRL B-12526]
VLTPIRSSKFKKDIKKLQKRGKDMEKLKKAILYLINEQALPPTYRNHILSGDWSGYYDLHLEPDWLLLYKITDMELLLAR
TGSHSDIFK

Nucleotide

• Length: 270 bp

>T44861 NZ_CP003712:11123-11392 [Zymomonas mobilis subsp. mobilis NRRL B-12526]
GTGCTAACGCCTATCCGATCGAGCAAATTCAAGAAAGACATAAAAAAATTACAGAAGCGAGGTAAGGATATGGAGAAATT
AAAAAAAGCCATTCTTTACCTGATTAATGAACAAGCCTTACCACCGACCTACCGAAACCATATTTTAAGTGGCGATTGGT
CGGGTTACTACGATTTGCATTTGGAACCTGACTGGCTTCTGCTCTATAAAATAACTGATATGGAATTGCTTCTAGCGCGA
ACAGGCAGCCATTCAGATATTTTTAAGTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9341.98 Da
• Isoelectric Point: 8.6589

>AT44861 WP_012954669.1 NZ_CP003712:10864-11121 [Zymomonas mobilis subsp. mobilis NRRL B-12526]
MKADSVVRARIPTLTKEAAIKTLEQMGLSISDAIRLLMTKIANEQRLPFSVELPTVETKKAIIELENGKGKAFKNADALF
EDLGI

Nucleotide

• Length: 258 bp

>AT44861 NZ_CP003712:10864-11121 [Zymomonas mobilis subsp. mobilis NRRL B-12526]
ATGAAAGCAGATTCTGTTGTTAGAGCACGCATCCCAACCTTGACCAAGGAAGCCGCGATTAAAACTTTGGAGCAGATGGG
ACTTAGTATTTCTGATGCTATCCGGTTGCTTATGACTAAAATCGCCAATGAGCAGCGCCTCCCCTTTTCTGTTGAATTAC
CAACGGTGGAAACCAAAAAAGCGATTATCGAATTGGAAAATGGAAAAGGTAAGGCTTTTAAAAATGCAGATGCTCTGTTC
GAGGATTTAGGTATTTAA

Structures

Toxin

• AlphaFold DB: A0A806D8N5

Antitoxin

• AlphaFold DB: A0A806DA69