Detailed information of TA system TA044857

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4476212..4476942
• Replicon: chromosome
• Accession: NZ_CP003424
• Organism: Serratia sp. SCBI

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A8B4GR63
• Locus tag: SERRSCBI_RS20895
• Protein ID: WP_004937033.1
• Coordinates: 4476212..4476523 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: SERRSCBI_RS20900
• Protein ID: WP_033644489.1
• Coordinates: 4476520..4476942 (+)
• Length: 141 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SERRSCBI_RS20865	4471527..4472252	+	726	WP_042785437.1	LPS export ABC transporter ATP-binding protein	-
SERRSCBI_RS20870	4472305..4473738	+	1434	WP_004937039.1	RNA polymerase factor sigma-54	-
SERRSCBI_RS20875	4473762..4474049	+	288	WP_004937038.1	ribosome hibernation promoting factor	-
SERRSCBI_RS20880	4474205..4474666	+	462	WP_016930212.1	PTS IIA-like nitrogen regulatory protein PtsN	-
SERRSCBI_RS20885	4474794..4475648	+	855	WP_015379177.1	RNase adapter RapZ	-
SERRSCBI_RS20890	4475645..4475917	+	273	WP_004937034.1	PTS phosphocarrier protein NPr	-
SERRSCBI_RS20895	4476212..4476523	+	312	WP_004937033.1	type II toxin-antitoxin system HigB family toxin	Toxin
SERRSCBI_RS20900	4476520..4476942	+	423	WP_033644489.1	helix-turn-helix domain-containing protein	Antitoxin
SERRSCBI_RS20905	4476947..4478862	-	1916	Protein_4175	BglG family transcription antiterminator	-
SERRSCBI_RS20910	4478914..4480128	-	1215	WP_042785438.1	lactonase family protein	-
SERRSCBI_RS20915	4480414..4481154	-	741	WP_042785439.1	KDGP aldolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (81-135)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12289.14 Da
• Isoelectric Point: 10.0957

>T44857 WP_004937033.1 NZ_CP003424:4476212-4476523 [Serratia sp. SCBI]
MHIISREPFNNATKQYPNEATALEAIYKALRRGEFRTPDELKALFPSLDRMKYKEKWWVIDVGGNHLRILFFASFETQKV
FIKHIVSHAEYDKLMQHYRRSSS

Nucleotide

• Length: 312 bp

>T44857 NZ_CP003424:4476212-4476523 [Serratia sp. SCBI]
ATGCATATCATTTCCAGGGAACCGTTCAATAACGCAACGAAGCAATATCCCAATGAAGCCACTGCTCTCGAGGCCATTTA
TAAAGCGTTGCGACGGGGCGAGTTCCGCACGCCGGACGAGTTAAAAGCGCTTTTTCCTTCGCTCGATCGTATGAAGTACA
AAGAGAAATGGTGGGTCATTGACGTCGGCGGCAACCACCTGCGGATCTTGTTCTTTGCCAGCTTCGAGACACAGAAAGTC
TTCATAAAGCATATCGTTAGCCACGCCGAATACGACAAGCTGATGCAGCACTATCGAAGGAGTTCGTCATGA

Antitoxin

Protein

• Length: 141 a.a.
• Molecular weight: 15784.13 Da
• Isoelectric Point: 5.2139

>AT44857 WP_033644489.1 NZ_CP003424:4476520-4476942 [Serratia sp. SCBI]
MITDAIKAADALLRAVPLLQGSHAQQDYREALELVEYLLENDERHPLIDMLAKKIAEYEDNSAEFTAFNQRIAQLPQGVA
ALRVLMEQHQLSQSDFEHEIGKKSLVNQILNGKRSLTIPHIMALAKRFNLPPALFLPEAD

Nucleotide

• Length: 423 bp

>AT44857 NZ_CP003424:4476520-4476942 [Serratia sp. SCBI]
ATGATTACAGATGCCATTAAGGCGGCCGATGCGCTACTGCGCGCCGTACCCTTATTGCAGGGCAGCCACGCTCAGCAAGA
CTACCGAGAAGCGCTCGAATTGGTGGAATATCTGCTGGAAAACGATGAACGCCATCCGCTGATTGACATGTTGGCAAAAA
AAATCGCCGAATATGAAGACAACAGCGCCGAATTTACCGCATTCAACCAGCGTATCGCCCAACTCCCACAGGGGGTGGCC
GCGCTTCGCGTATTGATGGAACAGCACCAACTCAGCCAAAGTGATTTTGAACATGAGATCGGCAAAAAGTCGTTGGTCAA
CCAAATCCTGAACGGCAAAAGATCGCTGACCATTCCGCACATTATGGCGCTGGCCAAACGCTTCAATCTGCCCCCAGCCC
TATTCCTGCCGGAAGCGGATTAG

Structures

Toxin

• AlphaFold DB: A0A8B4GR63