Detailed information of TA system TA044836

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1589820..1590425
• Replicon: chromosome
• Accession: NZ_CP003181
• Organism: Granulibacter bethesdensis strain NIH3.1

Toxin (Protein)

• Gene name: graT
• Uniprot ID: W6IIM5
• Locus tag: GBCGDNIH3_RS07365
• Protein ID: WP_025286830.1
• Coordinates: 1590147..1590425 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GBCGDNIH3_RS07360
• Protein ID: WP_025286829.1
• Coordinates: 1589820..1590134 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GBCGDNIH3_RS07335	1587187..1587531	+	345	WP_025286825.1	transcriptional regulator	-
GBCGDNIH3_RS07345	1588061..1588813	+	753	WP_025286826.1	phage antirepressor KilAC domain-containing protein	-
GBCGDNIH3_RS07350	1588813..1589184	+	372	WP_025286827.1	hypothetical protein	-
GBCGDNIH3_RS07355	1589225..1589467	-	243	WP_025286828.1	type II toxin-antitoxin system HicB family antitoxin	-
GBCGDNIH3_RS07360	1589820..1590134	-	315	WP_025286829.1	HigA family addiction module antidote protein	Antitoxin
GBCGDNIH3_RS07365	1590147..1590425	-	279	WP_025286830.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GBCGDNIH3_RS07370	1590526..1592184	+	1659	WP_025286831.1	hypothetical protein	-
GBCGDNIH3_RS07375	1592184..1592987	+	804	WP_025286832.1	hypothetical protein	-
GBCGDNIH3_RS07380	1593008..1594318	+	1311	WP_164504163.1	tail fiber domain-containing protein	-
GBCGDNIH3_RS07385	1594337..1594831	+	495	WP_025286834.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1555282..1600470	45188

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (3-93)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10656.01 Da
• Isoelectric Point: 5.8535

>T44836 WP_025286830.1 NZ_CP003181:c1590425-1590147 [Granulibacter bethesdensis]
MIRSWADKEAEALFDTGRSRRWATIRKVIQRKLDMLDAATALDDLRSPPGNRLEMLSGDRAGQYSIRVNDQFRICFNWTE
AGPENVEIVDYH

Nucleotide

• Length: 279 bp

>T44836 NZ_CP003181:c1590425-1590147 [Granulibacter bethesdensis]
ATGATCAGGTCGTGGGCGGACAAAGAAGCAGAGGCGCTTTTCGACACCGGACGCTCAAGGCGATGGGCGACGATCAGGAA
AGTTATCCAGCGCAAGCTGGATATGCTTGATGCCGCCACCGCGCTGGATGATCTGCGATCCCCGCCAGGCAACCGGCTGG
AAATGCTGAGTGGGGACCGGGCGGGGCAATACAGCATCCGCGTGAACGATCAGTTTCGCATCTGTTTCAACTGGACCGAG
GCCGGGCCGGAGAATGTCGAAATCGTCGATTACCACTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11326.89 Da
• Isoelectric Point: 7.9421

>AT44836 WP_025286829.1 NZ_CP003181:c1590134-1589820 [Granulibacter bethesdensis]
MSRIRTHPGEILQEEFLIPLGLTANALARALHVPANRITAIIADQRGITADTALRLACYFGTTPQFWLNLQTAHDLSLAQ
AARGNDISRTITPHASSQPTQHCS

Nucleotide

• Length: 315 bp

>AT44836 NZ_CP003181:c1590134-1589820 [Granulibacter bethesdensis]
ATGAGTCGTATCAGAACCCATCCGGGAGAAATCCTGCAGGAAGAATTCCTGATCCCTTTGGGCCTGACCGCGAATGCGCT
GGCGCGCGCCCTGCACGTTCCGGCCAACCGCATCACCGCCATTATTGCCGACCAGCGCGGCATCACGGCTGATACTGCCC
TCAGGCTGGCCTGCTATTTCGGCACCACGCCGCAATTCTGGCTGAACCTGCAAACCGCCCATGATTTGTCGCTGGCACAG
GCGGCGCGGGGAAATGATATTTCGAGGACAATCACGCCGCACGCTTCCAGCCAGCCGACCCAACATTGCTCTTGA

Structures

Toxin

• AlphaFold DB: W6IIM5