Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 3546009..3546679 Replicon chromosome
Accession NZ_CP002885
Organism Mycobacterium tuberculosis CCDC5180

Toxin (Protein)


Gene name higB Uniprot ID O53332
Locus tag CFBR_RS16805 Protein ID WP_003899954.1
Coordinates 3546009..3546353 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0THF6
Locus tag CFBR_RS16810 Protein ID WP_003899955.1
Coordinates 3546350..3546679 (+) Length 110 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CFBR_RS16780 3541895..3543184 + 1290 WP_003416640.1 ATP-binding protein -
CFBR_RS16795 3544889..3545323 - 435 WP_003899952.1 type II toxin-antitoxin system VapC family toxin -
CFBR_RS16800 3545326..3545778 - 453 WP_003899953.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
CFBR_RS16805 3546009..3546353 + 345 WP_003899954.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CFBR_RS16810 3546350..3546679 + 330 WP_003899955.1 XRE family transcriptional regulator Antitoxin
CFBR_RS16815 3547217..3547564 + 348 WP_003899956.1 DUF2384 domain-containing protein -
CFBR_RS16820 3547561..3548181 + 621 WP_003899957.1 RES family NAD+ phosphorylase -
CFBR_RS16825 3548341..3549606 - 1266 WP_003902423.1 hypothetical protein -
CFBR_RS16830 3549775..3549984 + 210 WP_003416778.1 hypothetical protein -
CFBR_RS16840 3550231..3551265 - 1035 WP_003416786.1 IS30 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 3538366..3564437 26071


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-91)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12692.50 Da        Isoelectric Point: 5.6920

>T44830 WP_003899954.1 NZ_CP002885:3546009-3546353 [Mycobacterium tuberculosis CCDC5180]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Download         Length: 345 bp

>T44830 NZ_CP002885:3546009-3546353 [Mycobacterium tuberculosis CCDC5180]
TTGGCCGTGATCCTGCTCCCGCAGGTCGAACGGTGGTTCTTCGCGCTCAACAGGGATGCGATGGCCTCGGTCACCGGCGC
CATCGACCTGCTCGAAATGGAGGGGCCGACGTTGGGCCGCCCGGTGGTCGACAAAGTGAACGACTCAACGTTTCACAACA
TGAAGGAGCTGCGCCCCGCCGGCACCAGCATCCGGATCCTGTTCGCCTTCGACCCGGCCCGGCAGGCGATCCTGCTGCTG
GGCGGTGACAAGGCAGGCAACTGGAAACGCTGGTACGACAACAACATTCCAATCGCTGACCAGCGCTCCGAGAACTGGCT
GGCGAGCGAGCACGGAGGTGGATGA

Antitoxin


Download         Length: 110 a.a.        Molecular weight: 11802.46 Da        Isoelectric Point: 7.4051

>AT44830 WP_003899955.1 NZ_CP002885:3546350-3546679 [Mycobacterium tuberculosis CCDC5180]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Download         Length: 330 bp

>AT44830 NZ_CP002885:3546350-3546679 [Mycobacterium tuberculosis CCDC5180]
ATGACCATGGCCCGCAACTGGCGTGACATTCGCGCCGATGCCGTCGCGCAGGGCCGCGTGGATCTGCAGCGGGCGGCCGT
GGCACGCGAGGAGATGCGCGATGCCGTCCTGGCGCACCGCCTGGCCGAGATCCGCAAGGCGCTAGGCCACGCACGTCAGG
CCGACGTCGCGGCGCTGATGGGGGTCTCTCAGGCCCGTGTCTCCAAGCTGGAGAGCGGCGACCTGTCCCACACCGAACTC
GGCACCCTGCAGGCCTACGTTGCCGCCCTGGGCGGGCACCTGCGCATCGTCGCTGAGTTCGGCGAAAATACTGTCGAGCT
GACCGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FBK2

Antitoxin

Source ID Structure
PDB 6LTY
PDB 6LTZ
AlphaFold DB G0THF6

References