Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 2258730..2259649 Replicon chromosome
Accession NZ_CP002885
Organism Mycobacterium tuberculosis CCDC5180

Toxin (Protein)


Gene name HigB2 Uniprot ID L7N4R2
Locus tag CFBR_RS21410 Protein ID WP_003900449.1
Coordinates 2259044..2259649 (-) Length 202 a.a.

Antitoxin (Protein)


Gene name HigA2 Uniprot ID L7N5K9
Locus tag CFBR_RS10605 Protein ID WP_003410124.1
Coordinates 2258730..2259035 (-) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CFBR_RS10565 2253993..2254193 + 201 Protein_2074 hypothetical protein -
CFBR_RS10580 2255549..2255926 + 378 Protein_2076 hypothetical protein -
CFBR_RS10585 2255923..2256963 + 1041 WP_003410103.1 ImmA/IrrE family metallo-endopeptidase -
CFBR_RS10590 2257205..2257924 + 720 WP_003410108.1 DUF433 domain-containing protein -
CFBR_RS10595 2257914..2258330 + 417 WP_003410114.1 hypothetical protein -
CFBR_RS10600 2258346..2258645 - 300 WP_003410120.1 hypothetical protein -
CFBR_RS10605 2258730..2259035 - 306 WP_003410124.1 XRE family transcriptional regulator Antitoxin
CFBR_RS21410 2259044..2259649 - 606 WP_003900449.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CFBR_RS10615 2259674..2260033 - 360 WP_003410131.1 hypothetical protein -
CFBR_RS10620 2260193..2260870 - 678 WP_003905725.1 hypothetical protein -
CFBR_RS10625 2260822..2261058 - 237 WP_003410133.1 hypothetical protein -
CFBR_RS10630 2261265..2262161 - 897 WP_003410136.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(86-181)

Antitoxin

HigA2-like, helix-turn-helix domain

(32-92)


Sequences

Toxin        


Download         Length: 202 a.a.        Molecular weight: 22744.96 Da        Isoelectric Point: 7.3073

>T44812 WP_003900449.1 NZ_CP002885:c2259649-2259044 [Mycobacterium tuberculosis CCDC5180]
VNVPWENAHGGALYCLIRGDEFSAWHRLLFQRPGCAESVLACRHFLDGSPVARCSYPEEYHPCVISRIALLCDSVGWTAD
VERISAWLNGLDRETYELVFAAIEVLEEEGPALGCPLVDTVRGSRHKNMKELRPGSQGRSEVRILFAFDPARQAIMLAAG
NKAGRWTQWYDEKIKAADEMFAEHLAQFEDTKPKRRKRKKG

Download         Length: 606 bp

>T44812 NZ_CP002885:c2259649-2259044 [Mycobacterium tuberculosis CCDC5180]
GTGAACGTTCCATGGGAGAACGCGCACGGCGGCGCACTGTACTGCCTCATACGTGGGGATGAATTCTCGGCCTGGCACAG
GCTACTGTTTCAACGGCCCGGGTGCGCAGAGTCCGTCCTCGCCTGCCGGCATTTCCTCGACGGTAGCCCCGTCGCCCGCT
GCTCTTACCCGGAGGAATATCACCCTTGTGTGATATCGCGTATCGCGCTACTCTGCGACTCTGTGGGGTGGACGGCGGAT
GTGGAGCGCATCTCCGCCTGGCTCAACGGCTTGGACCGAGAGACCTACGAATTGGTGTTCGCAGCTATCGAGGTTCTCGA
GGAAGAGGGCCCCGCACTTGGCTGTCCTCTGGTGGACACGGTGCGTGGGTCGCGCCACAAGAACATGAAGGAGCTGCGTC
CAGGATCACAAGGGCGGTCCGAGGTCCGCATTCTGTTCGCCTTCGACCCGGCGCGGCAGGCGATCATGCTGGCCGCCGGT
AACAAGGCCGGGCGGTGGACCCAGTGGTACGACGAGAAGATCAAAGCGGCCGATGAGATGTTTGCCGAACATCTCGCACA
GTTCGAGGACACGAAGCCAAAGAGGCGCAAGCGCAAGAAAGGGTAA

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11291.91 Da        Isoelectric Point: 10.7482

>AT44812 WP_003410124.1 NZ_CP002885:c2259035-2258730 [Mycobacterium tuberculosis CCDC5180]
MAMTLRDMDAVRPVNREAVDRHKARMRDEVRAFRLRELRAAQSLTQVQVAALAHIRQSRVSSIENGDIGSAQVNTLRKYV
SALGGELDITVRLGDETFTLA

Download         Length: 306 bp

>AT44812 NZ_CP002885:c2259035-2258730 [Mycobacterium tuberculosis CCDC5180]
ATGGCGATGACACTACGGGACATGGACGCCGTACGTCCCGTTAACCGGGAGGCGGTGGACCGGCACAAGGCACGAATGCG
CGATGAAGTGCGGGCGTTCCGGCTCCGGGAGCTGCGCGCGGCTCAGTCACTCACCCAGGTCCAGGTGGCTGCGCTGGCGC
ACATCCGGCAGAGCCGGGTATCCAGCATCGAGAACGGCGACATCGGCTCCGCACAGGTCAACACACTGCGCAAATACGTG
TCCGCTCTTGGCGGAGAACTGGATATCACCGTCCGCCTGGGAGATGAGACATTCACCCTGGCATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BV20

Antitoxin

Source ID Structure
PDB 7EWC
PDB 7EWD
PDB 7EWE
AlphaFold DB A0A7U4BV30

References