Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) higBA (relBE)/Gp49-HTH_37
Location 2187791..2188659 Replicon chromosome
Accession NZ_CP002885
Organism Mycobacterium tuberculosis CCDC5180

Toxin (Protein)


Gene name higB Uniprot ID P9WJA4
Locus tag CFBR_RS10185 Protein ID WP_010886136.1
Coordinates 2187791..2188168 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0TLM0
Locus tag CFBR_RS10190 Protein ID WP_003409886.1
Coordinates 2188210..2188659 (+) Length 150 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CFBR_RS10135 2183580..2184032 - 453 WP_003899095.1 lipoprotein -
CFBR_RS10140 2184096..2184497 + 402 WP_003409869.1 hypothetical protein -
CFBR_RS10145 2184490..2184672 - 183 WP_003409870.1 hypothetical protein -
CFBR_RS10150 2184786..2185136 - 351 WP_003409871.1 hypothetical protein -
CFBR_RS10155 2185147..2186049 - 903 WP_003899097.1 hypothetical protein -
CFBR_RS10160 2186070..2186261 - 192 WP_003409876.1 hypothetical protein -
CFBR_RS10165 2186262..2186558 - 297 WP_003409877.1 hypothetical protein -
CFBR_RS10170 2186798..2187013 + 216 WP_003409878.1 antitoxin -
CFBR_RS10175 2187010..2187321 + 312 WP_003409881.1 type II toxin-antitoxin system VapC family toxin -
CFBR_RS22600 2187295..2187816 - 522 WP_003904745.1 hypothetical protein -
CFBR_RS10185 2187791..2188168 + 378 WP_010886136.1 type II toxin-antitoxin system toxin HigB Toxin
CFBR_RS10190 2188210..2188659 + 450 WP_003409886.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
CFBR_RS10195 2188656..2189201 + 546 WP_003409891.1 SecB-like chaperone -
CFBR_RS10200 2189090..2189704 - 615 WP_003901296.1 hypothetical protein -
CFBR_RS10205 2189753..2190049 - 297 WP_003409896.1 type II toxin-antitoxin system RelE/ParE family toxin -
CFBR_RS10210 2190046..2190297 - 252 WP_003409899.1 type II toxin-antitoxin system ParD family antitoxin -
CFBR_RS10215 2190284..2190778 + 495 WP_003899099.1 hypothetical protein -
CFBR_RS10220 2190938..2191345 - 408 WP_003409913.1 type II toxin-antitoxin system VapC family toxin -
CFBR_RS10225 2191349..2191621 - 273 WP_003899100.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
CFBR_RS10230 2191654..2192874 - 1221 WP_003409919.1 TetR family transcriptional regulator Mce3R -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(27-113)

Antitoxin

(44-91)


Sequences


Toxin        


Download         Length: 126 a.a.        Molecular weight: 14397.77 Da        Isoelectric Point: 10.8862

>T44806 WP_010886136.1 NZ_CP002885:2187791-2188168 [Mycobacterium tuberculosis CCDC5180]
VPPPDPAAMGTWKFFRASVDGRPVFKKEFDKLPDQARAALIVLMQRYLVGDLAAGSIKPIRGDILELRWHEANNHFRVLF
FRWGQHPVALTAFYKNQQKTPKTKIETALDRQKIWKRAFGDTPPI

Download         Length: 378 bp

>T44806 NZ_CP002885:2187791-2188168 [Mycobacterium tuberculosis CCDC5180]
GTGCCGCCCCCTGATCCAGCCGCCATGGGTACCTGGAAGTTCTTCCGGGCATCTGTGGATGGCCGGCCGGTATTCAAGAA
GGAGTTCGACAAGCTTCCTGATCAGGCCCGGGCCGCGCTGATCGTGCTAATGCAGCGGTATCTCGTCGGCGACCTCGCCG
CAGGGAGCATCAAACCGATTCGTGGCGACATTCTGGAGTTGCGATGGCATGAGGCGAACAACCACTTCCGGGTACTGTTC
TTCCGCTGGGGCCAGCATCCCGTAGCGCTGACAGCGTTCTACAAGAACCAGCAGAAGACTCCCAAGACGAAGATCGAGAC
GGCCCTGGACCGGCAGAAAATCTGGAAAAGAGCCTTCGGCGACACCCCACCGATCTGA

Antitoxin


Download         Length: 150 a.a.        Molecular weight: 16760.16 Da        Isoelectric Point: 8.0771

>AT44806 WP_003409886.1 NZ_CP002885:2188210-2188659 [Mycobacterium tuberculosis CCDC5180]
MSIDFPLGDDLAGYIAEAIAADPSFKGTLEDAEEARRLVDALIALRKHCQLSQVEVAKRMGVRQPTVSGFEKEPSDPKLS
TLQRYARALDARLRLVLEVPTLREVPTWHRLSSYRGSARDHQVRVGADKEILMQTNWARHISVRQVEVA

Download         Length: 450 bp

>AT44806 NZ_CP002885:2188210-2188659 [Mycobacterium tuberculosis CCDC5180]
ATGAGCATTGACTTCCCTTTGGGTGACGACCTCGCCGGCTATATTGCCGAGGCGATTGCGGCTGATCCCAGCTTCAAAGG
CACTCTCGAAGACGCCGAGGAGGCACGCAGGCTGGTCGATGCGCTGATTGCGCTGCGCAAGCACTGCCAGCTGAGCCAGG
TTGAGGTTGCTAAGCGTATGGGGGTGCGCCAGCCCACCGTGAGCGGTTTCGAGAAGGAACCCAGCGACCCCAAACTGTCT
ACGCTGCAACGTTATGCCCGTGCATTGGACGCCCGGCTGCGGCTGGTGCTCGAAGTTCCCACGCTTCGCGAAGTGCCTAC
GTGGCATCGGCTCTCCTCTTATCGGGGCTCCGCACGGGACCATCAGGTCCGGGTGGGTGCAGACAAGGAAATCCTGATGC
AGACGAACTGGGCCCGCCACATTTCGGTTCGGCAGGTTGAGGTGGCATGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
PDB 7AWK
PDB 7NBU


Antitoxin

Source ID Structure
PDB 5MTW
AlphaFold DB G0TLM0

References