Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 3539417..3540087 Replicon chromosome
Accession NZ_CP002883
Organism Mycobacterium tuberculosis BT1

Toxin (Protein)


Gene name higB Uniprot ID O53332
Locus tag HKBT1_RS16750 Protein ID WP_003899954.1
Coordinates 3539417..3539761 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0THF6
Locus tag HKBT1_RS16755 Protein ID WP_003899955.1
Coordinates 3539758..3540087 (+) Length 110 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HKBT1_RS16720 3534722..3535015 + 294 WP_003416635.1 hypothetical protein -
HKBT1_RS16725 3535303..3536592 + 1290 WP_003416640.1 ATP-binding protein -
HKBT1_RS16740 3538297..3538731 - 435 WP_003899952.1 type II toxin-antitoxin system VapC family toxin -
HKBT1_RS16745 3538734..3539186 - 453 WP_003899953.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
HKBT1_RS16750 3539417..3539761 + 345 WP_003899954.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HKBT1_RS16755 3539758..3540087 + 330 WP_003899955.1 XRE family transcriptional regulator Antitoxin
HKBT1_RS16760 3540625..3540972 + 348 WP_003899956.1 DUF2384 domain-containing protein -
HKBT1_RS16765 3540969..3541589 + 621 WP_003899957.1 RES family NAD+ phosphorylase -
HKBT1_RS16770 3541749..3543014 - 1266 WP_003902423.1 hypothetical protein -
HKBT1_RS16775 3543182..3543391 + 210 WP_003416778.1 hypothetical protein -
HKBT1_RS16785 3543638..3544672 - 1035 WP_003416786.1 IS30 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-91)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12692.50 Da        Isoelectric Point: 5.6920

>T44770 WP_003899954.1 NZ_CP002883:3539417-3539761 [Mycobacterium tuberculosis BT1]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Download         Length: 345 bp

>T44770 NZ_CP002883:3539417-3539761 [Mycobacterium tuberculosis BT1]
TTGGCCGTGATCCTGCTCCCGCAGGTCGAACGGTGGTTCTTCGCGCTCAACAGGGATGCGATGGCCTCGGTCACCGGCGC
CATCGACCTGCTCGAAATGGAGGGGCCGACGTTGGGCCGCCCGGTGGTCGACAAAGTGAACGACTCAACGTTTCACAACA
TGAAGGAGCTGCGCCCCGCCGGCACCAGCATCCGGATCCTGTTCGCCTTCGACCCGGCCCGGCAGGCGATCCTGCTGCTG
GGCGGTGACAAGGCAGGCAACTGGAAACGCTGGTACGACAACAACATTCCAATCGCTGACCAGCGCTCCGAGAACTGGCT
GGCGAGCGAGCACGGAGGTGGATGA

Antitoxin


Download         Length: 110 a.a.        Molecular weight: 11802.46 Da        Isoelectric Point: 7.4051

>AT44770 WP_003899955.1 NZ_CP002883:3539758-3540087 [Mycobacterium tuberculosis BT1]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Download         Length: 330 bp

>AT44770 NZ_CP002883:3539758-3540087 [Mycobacterium tuberculosis BT1]
ATGACCATGGCCCGCAACTGGCGTGACATTCGCGCCGATGCCGTCGCGCAGGGCCGCGTGGATCTGCAGCGGGCGGCCGT
GGCACGCGAGGAGATGCGCGATGCCGTCCTGGCGCACCGCCTGGCCGAGATCCGCAAGGCGCTAGGCCACGCACGTCAGG
CCGACGTCGCGGCGCTGATGGGGGTCTCTCAGGCCCGTGTCTCCAAGCTGGAGAGCGGCGACCTGTCCCACACCGAACTC
GGCACCCTGCAGGCCTACGTTGCCGCCCTGGGCGGGCACCTGCGCATCGTCGCTGAGTTCGGCGAAAATACTGTCGAGCT
GACCGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FBK2

Antitoxin

Source ID Structure
PDB 6LTY
PDB 6LTZ
AlphaFold DB G0THF6

References