Detailed information of TA system TA044563

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 2702552..2703150
• Replicon: chromosome
• Accession: NZ_AP028128
• Organism: Vibrio fluvialis strain IDH05335

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7Z7VLH5
• Locus tag: QUF32_RS12750
• Protein ID: WP_054103799.1
• Coordinates: 2702552..2702875 (+)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: QUF32_RS12755
• Protein ID: WP_000058216.1
• Coordinates: 2702872..2703150 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUF32_RS12720 (KKIDH5335_24400)	2698265..2698651	-	387	WP_000180225.1	TraA family conjugative transfer protein	-
QUF32_RS12725	2698648..2699220	-	573	WP_148039504.1	type IV conjugative transfer system lipoprotein TraV	-
QUF32_RS12730 (KKIDH5335_24410)	2699295..2700584	-	1290	WP_206989652.1	TraB/VirB10 family protein	-
QUF32_RS12735 (KKIDH5335_24420)	2700587..2701483	-	897	WP_286236290.1	type-F conjugative transfer system secretin TraK	-
QUF32_RS12740 (KKIDH5335_24430)	2701467..2702093	-	627	WP_064497042.1	TraE/TraK family type IV conjugative transfer system protein	-
QUF32_RS12745 (KKIDH5335_24440)	2702090..2702371	-	282	WP_000433891.1	type IV conjugative transfer system protein TraL	-
QUF32_RS12750 (KKIDH5335_24450)	2702552..2702875	+	324	WP_054103799.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
QUF32_RS12755 (KKIDH5335_24460)	2702872..2703150	+	279	WP_000058216.1	helix-turn-helix transcriptional regulator	Antitoxin
QUF32_RS12760 (KKIDH5335_24470)	2703176..2703811	-	636	WP_000033757.1	DUF4400 domain-containing protein	-
QUF32_RS12765 (KKIDH5335_24480)	2703798..2704358	-	561	WP_286236246.1	conjugative transfer protein	-
QUF32_RS12770 (KKIDH5335_24490)	2704368..2706188	-	1821	WP_286236247.1	conjugative transfer system coupling protein TraD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	2593479..2747589	154110

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (15-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (31-81)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 12295.35 Da
• Isoelectric Point: 9.7658

>T44563 WP_054103799.1 NZ_AP028128:2702552-2702875 [Vibrio fluvialis]
MSWKIDFYDGVEEQILDMPPKIQARMIKLLELMEKHGANLGPPHTESMGDGLFEIRAKAQEGIGRGLFCYLKGKHIYVLH
AFVKKSQKTPKNELNLARDRQKEVQKS

Nucleotide

• Length: 324 bp

>T44563 NZ_AP028128:2702552-2702875 [Vibrio fluvialis]
ATGAGCTGGAAGATCGACTTTTACGATGGCGTTGAGGAGCAGATCCTCGATATGCCCCCCAAAATACAAGCTCGCATGAT
TAAGCTTTTAGAGCTGATGGAAAAGCATGGTGCAAACTTGGGGCCTCCTCATACTGAGTCTATGGGCGATGGGTTATTTG
AAATCCGCGCCAAAGCTCAAGAAGGTATAGGTAGAGGCTTATTTTGCTACTTAAAAGGGAAGCACATTTATGTGTTACAT
GCTTTTGTAAAAAAATCTCAAAAGACGCCCAAAAATGAGCTTAATCTTGCCAGAGATAGACAAAAAGAGGTTCAAAAATC
ATGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10517.07 Da
• Isoelectric Point: 9.9393

>AT44563 WP_000058216.1 NZ_AP028128:2702872-2703150 [Vibrio fluvialis]
MSLQKLKQRALANAEVKNEYDKLESEFSFIDQLLTMRTKAGLTQEQVAERMHTQKSNVSRLEKGNANPSWSTLLKYAHAC
GFELTLKPQKMS

Nucleotide

• Length: 279 bp

>AT44563 NZ_AP028128:2702872-2703150 [Vibrio fluvialis]
ATGAGTCTGCAAAAACTGAAACAACGTGCTTTAGCCAATGCTGAAGTTAAGAATGAGTACGATAAGCTTGAGAGTGAGTT
CAGCTTTATTGACCAATTGCTTACCATGAGAACAAAGGCAGGACTTACTCAAGAACAAGTTGCTGAAAGAATGCATACCC
AAAAAAGTAATGTCAGTCGTTTAGAAAAAGGTAATGCCAATCCAAGTTGGTCAACTCTTCTAAAATATGCTCACGCATGT
GGCTTTGAACTAACACTCAAGCCACAAAAAATGAGTTAA

Structures

Toxin

• AlphaFold DB: A0A7Z7VLH5