Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type I Classification (family/domain) hok-sok/-
Location 74804..75057 Replicon plasmid pJNE132847_2
Accession NZ_AP028096
Organism Escherichia coli strain JNE132847

Toxin (Protein)


Gene name srnB Uniprot ID G9G1E3
Locus tag JE76HU_RS27175 Protein ID WP_001312851.1
Coordinates 74908..75057 (+) Length 50 a.a.

Antitoxin (RNA)


Gene name sok
Locus tag -
Coordinates 74804..74863 (-)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JE76HU_RS27145 (69861) 69861..70766 - 906 WP_000219391.1 Mph(A) family macrolide 2'-phosphotransferase -
JE76HU_RS27150 (70888) 70888..71592 - 705 WP_001067855.1 IS6-like element IS26 family transposase -
JE76HU_RS27155 (71639) 71639..71815 - 177 WP_200922282.1 DUF4158 domain-containing protein -
JE76HU_RS27160 (71979) 71979..72536 + 558 WP_001235713.1 recombinase family protein -
JE76HU_RS27165 (72719) 72719..73579 + 861 WP_000027057.1 broad-spectrum class A beta-lactamase TEM-1 -
JE76HU_RS27170 (74386) 74386..74661 + 276 WP_072276260.1 hypothetical protein -
- (74804) 74804..74863 - 60 NuclAT_1 - Antitoxin
- (74804) 74804..74863 - 60 NuclAT_1 - Antitoxin
- (74804) 74804..74863 - 60 NuclAT_1 - Antitoxin
- (74804) 74804..74863 - 60 NuclAT_1 - Antitoxin
JE76HU_RS27175 (74908) 74908..75057 + 150 WP_001312851.1 Hok/Gef family protein Toxin
JE76HU_RS27180 (75342) 75342..75590 + 249 WP_000083839.1 replication regulatory protein RepA -
JE76HU_RS27185 (75705) 75705..75889 + 185 Protein_93 protein CopA/IncA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid mph(A) / blaTEM-1B - 1..75901 75901


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Hok/gef cell toxic protein

(4-45)


Sequences

Toxin        


Download         Length: 50 a.a.        Molecular weight: 5542.67 Da        Isoelectric Point: 8.7678

>T44535 WP_001312851.1 NZ_AP028096:74908-75057 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Download         Length: 150 bp

>T44535 NZ_AP028096:74908-75057 [Escherichia coli]
ATGACGAAGTATGCCCTTATCGGGTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAGCTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 60 bp

>AT44535 NZ_AP028096:c74863-74804 [Escherichia coli]
AATGTACTTCATGCAGACCTCACGAGTTAATGGATTAACAAGTGGGGTCTTTGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829C8K5

Antitoxin

Download structure file

References