Detailed information of TA system TA044521

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4586738..4587340
• Replicon: chromosome
• Accession: NZ_AP028094
• Organism: Escherichia coli strain JNE132847

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: JE76HU_RS23165
• Protein ID: WP_000897305.1
• Coordinates: 4586738..4587049 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JE76HU_RS23170
• Protein ID: WP_000356397.1
• Coordinates: 4587050..4587340 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JE76HU_RS23140 (4582652)	4582652..4583251	+	600	WP_001295269.1	glucose-1-phosphatase	-
JE76HU_RS23145 (4583245)	4583245..4584117	+	873	WP_000920762.1	virulence factor BrkB family protein	-
JE76HU_RS23150 (4584114)	4584114..4584551	+	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
JE76HU_RS23155 (4584596)	4584596..4585537	+	942	WP_001343389.1	fatty acid biosynthesis protein FabY	-
JE76HU_RS23160 (4585601)	4585601..4586509	-	909	WP_001162704.1	alpha/beta hydrolase	-
JE76HU_RS23165 (4586738)	4586738..4587049	+	312	WP_000897305.1	hypothetical protein	Toxin
JE76HU_RS23170 (4587050)	4587050..4587340	+	291	WP_000356397.1	NadS family protein	Antitoxin
JE76HU_RS23175 (4587945)	4587945..4588163	+	219	WP_001295676.1	CopG family transcriptional regulator	-
JE76HU_RS23180 (4588382)	4588382..4588624	+	243	WP_001086388.1	protein YiiF	-
JE76HU_RS23185 (4588853)	4588853..4589833	-	981	WP_000399676.1	IS110-like element IS621 family transposase	-
JE76HU_RS23190 (4590232)	4590232..4591161	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
JE76HU_RS23195 (4591158)	4591158..4591793	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	4588853..4589833	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T44521 WP_000897305.1 NZ_AP028094:4586738-4587049 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T44521 NZ_AP028094:4586738-4587049 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT44521 WP_000356397.1 NZ_AP028094:4587050-4587340 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT44521 NZ_AP028094:4587050-4587340 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7