Detailed information of TA system TA044496

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ-RelB
• Location: 280876..281417
• Replicon: chromosome
• Accession: NZ_AP028094
• Organism: Escherichia coli strain JNE132847

Toxin (Protein)

• Gene name: yafQ
• Uniprot ID: U9XPG0
• Locus tag: JE76HU_RS01250
• Protein ID: WP_000615976.1
• Coordinates: 280876..281154 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: -
• Locus tag: JE76HU_RS01255
• Protein ID: WP_024216090.1
• Coordinates: 281157..281417 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JE76HU_RS01235 (278458)	278458..279036	+	579	WP_000284050.1	D-sedoheptulose 7-phosphate isomerase	-
JE76HU_RS01240 (279242)	279242..280009	+	768	WP_000333380.1	class II glutamine amidotransferase	-
JE76HU_RS01245 (279980)	279980..280720	-	741	WP_001225679.1	peptidoglycan meso-diaminopimelic acid protein amidase	-
JE76HU_RS01250 (280876)	280876..281154	-	279	WP_000615976.1	type II toxin-antitoxin system mRNA interferase toxin YafQ	Toxin
JE76HU_RS01255 (281157)	281157..281417	-	261	WP_024216090.1	type II toxin-antitoxin system antitoxin DinJ	Antitoxin
JE76HU_RS01260 (281603)	281603..282376	+	774	WP_000543897.1	C40 family peptidase	-
JE76HU_RS01265 (282433)	282433..282789	-	357	WP_001030486.1	type II toxin-antitoxin system HicB family antitoxin	-
JE76HU_RS01270 (282782)	282782..283060	-	279	WP_024216089.1	type II toxin-antitoxin system HicA family toxin	-
JE76HU_RS01275 (283165)	283165..284877	-	1713	Protein_247	flagellar biosynthesis protein FlhA	-
JE76HU_RS01280 (284849)	284849..285634	+	786	WP_000207574.1	putative lateral flagellar export/assembly protein LafU	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (1-91)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-85)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10800.59 Da
• Isoelectric Point: 10.0702

>T44496 WP_000615976.1 NZ_AP028094:c281154-280876 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Nucleotide

• Length: 279 bp

>T44496 NZ_AP028094:c281154-280876 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9490.91 Da
• Isoelectric Point: 5.8275

>AT44496 WP_024216090.1 NZ_AP028094:c281417-281157 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLARMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Nucleotide

• Length: 261 bp

>AT44496 NZ_AP028094:c281417-281157 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCCGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Structures

Toxin

• AlphaFold DB: U9XPG0