Detailed information of TA system TA044436

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 1974157..1974815
• Replicon: chromosome
• Accession: NZ_AP028050
• Organism: Phytobacter diazotrophicus strain TA9832

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: QUE83_RS09235
• Protein ID: WP_125124251.1
• Coordinates: 1974157..1974510 (+)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A348DBJ5
• Locus tag: QUE83_RS09240
• Protein ID: WP_071196363.1
• Coordinates: 1974513..1974815 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUE83_RS09220 (1970611)	1970611..1972065	-	1455	WP_041851859.1	AMP nucleosidase	-
QUE83_RS09225 (1972259)	1972259..1973188	-	930	WP_232034419.1	MDR family oxidoreductase	-
QUE83_RS09230 (1973118)	1973118..1973938	-	821	Protein_1805	TetR family transcriptional regulator C-terminal domain-containing protein	-
QUE83_RS09235 (1974157)	1974157..1974510	+	354	WP_125124251.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
QUE83_RS09240 (1974513)	1974513..1974815	+	303	WP_071196363.1	XRE family transcriptional regulator	Antitoxin
QUE83_RS09245 (1974892)	1974892..1975842	+	951	WP_041851855.1	L,D-transpeptidase	-
QUE83_RS09250 (1975935)	1975935..1976123	-	189	WP_041851854.1	YjfB family protein	-
QUE83_RS09260 (1976721)	1976721..1977638	+	918	WP_041851853.1	nitrogen assimilation transcriptional regulator NAC	-
QUE83_RS09265 (1977740)	1977740..1978690	+	951	WP_041851852.1	HTH-type transcriptional regulator Cbl	-
QUE83_RS09270 (1978742)	1978742..1979218	-	477	WP_071196365.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1934201..2033606	99405

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-81)

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 13585.60 Da
• Isoelectric Point: 9.4816

>T44436 WP_125124251.1 NZ_AP028050:1974157-1974510 [Phytobacter diazotrophicus]
VWEIKTTDRFDEWLIAQNDHTRASVLAALIVLRTKGPELPRPWADTIKGARYSNMKELRIQCRGEPIRPFFAFDPRRAAI
VLCAGSKAGNEKRFYAEMISRAEQEYANYLKKPEKEE

Nucleotide

• Length: 354 bp

>T44436 NZ_AP028050:1974157-1974510 [Phytobacter diazotrophicus]
GTGTGGGAAATTAAAACAACGGACAGGTTTGATGAGTGGTTAATCGCTCAAAATGATCACACGCGTGCGAGCGTTTTGGC
GGCGCTGATCGTGTTGCGAACGAAAGGCCCCGAATTGCCCAGACCCTGGGCCGACACGATAAAAGGGGCGCGCTACAGCA
ATATGAAGGAGCTGCGAATTCAGTGTCGTGGGGAGCCGATACGCCCTTTTTTCGCTTTTGATCCCAGACGGGCGGCTATT
GTGTTGTGCGCAGGAAGTAAAGCTGGAAATGAAAAACGTTTCTACGCCGAAATGATTTCGCGAGCTGAGCAGGAATATGC
AAACTATCTGAAAAAACCAGAGAAAGAGGAGTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11067.80 Da
• Isoelectric Point: 6.2312

>AT44436 WP_071196363.1 NZ_AP028050:1974513-1974815 [Phytobacter diazotrophicus]
MGRTLEQLLADEKPEVVAQAESMATETLLNIHLAELREKVQKTQVEMAHALGIRQPTVAGMEKPGRDVKLSTLKRYVEAT
GGKLRLDIELPDGSHYAFVV

Nucleotide

• Length: 303 bp

>AT44436 NZ_AP028050:1974513-1974815 [Phytobacter diazotrophicus]
ATGGGCAGAACGCTGGAACAACTGCTTGCGGATGAAAAACCAGAGGTTGTGGCGCAGGCTGAGTCAATGGCGACTGAGAC
TCTCCTGAACATTCACCTGGCCGAACTGCGTGAGAAAGTGCAAAAAACGCAGGTGGAAATGGCGCATGCACTGGGGATAC
GGCAACCTACGGTGGCGGGAATGGAAAAACCGGGTCGCGATGTAAAACTGTCGACGCTTAAGCGCTACGTTGAAGCCACT
GGCGGTAAGCTTCGTCTTGATATCGAACTGCCTGATGGGTCGCATTACGCCTTCGTTGTATAA

Structures

Antitoxin

• AlphaFold DB: A0A348DBJ5