Detailed information of TA system TA044416

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 1975273..1975931
• Replicon: chromosome
• Accession: NZ_AP028047
• Organism: Phytobacter diazotrophicus strain TA9759

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: QUE80_RS09245
• Protein ID: WP_125124251.1
• Coordinates: 1975273..1975626 (+)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A348DBJ5
• Locus tag: QUE80_RS09250
• Protein ID: WP_071196363.1
• Coordinates: 1975629..1975931 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUE80_RS09230 (1971727)	1971727..1973181	-	1455	WP_041851859.1	AMP nucleosidase	-
QUE80_RS09235 (1973375)	1973375..1974304	-	930	WP_232034419.1	MDR family oxidoreductase	-
QUE80_RS09240 (1974234)	1974234..1975054	-	821	Protein_1807	TetR family transcriptional regulator C-terminal domain-containing protein	-
QUE80_RS09245 (1975273)	1975273..1975626	+	354	WP_125124251.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
QUE80_RS09250 (1975629)	1975629..1975931	+	303	WP_071196363.1	XRE family transcriptional regulator	Antitoxin
QUE80_RS09255 (1976008)	1976008..1976958	+	951	WP_041851855.1	L,D-transpeptidase	-
QUE80_RS09260 (1977051)	1977051..1977239	-	189	WP_041851854.1	YjfB family protein	-
QUE80_RS09270 (1977837)	1977837..1978754	+	918	WP_041851853.1	nitrogen assimilation transcriptional regulator NAC	-
QUE80_RS09275 (1978856)	1978856..1979806	+	951	WP_041851852.1	HTH-type transcriptional regulator Cbl	-
QUE80_RS09280 (1979858)	1979858..1980334	-	477	WP_071196365.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1973375..2034722	61347

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-81)

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 13585.60 Da
• Isoelectric Point: 9.4816

>T44416 WP_125124251.1 NZ_AP028047:1975273-1975626 [Phytobacter diazotrophicus]
VWEIKTTDRFDEWLIAQNDHTRASVLAALIVLRTKGPELPRPWADTIKGARYSNMKELRIQCRGEPIRPFFAFDPRRAAI
VLCAGSKAGNEKRFYAEMISRAEQEYANYLKKPEKEE

Nucleotide

• Length: 354 bp

>T44416 NZ_AP028047:1975273-1975626 [Phytobacter diazotrophicus]
GTGTGGGAAATTAAAACAACGGACAGGTTTGATGAGTGGTTAATCGCTCAAAATGATCACACGCGTGCGAGCGTTTTGGC
GGCGCTGATCGTGTTGCGAACGAAAGGCCCCGAATTGCCCAGACCCTGGGCCGACACGATAAAAGGGGCGCGCTACAGCA
ATATGAAGGAGCTGCGAATTCAGTGTCGTGGGGAGCCGATACGCCCTTTTTTCGCTTTTGATCCCAGACGGGCGGCTATT
GTGTTGTGCGCAGGAAGTAAAGCTGGAAATGAAAAACGTTTCTACGCCGAAATGATTTCGCGAGCTGAGCAGGAATATGC
AAACTATCTGAAAAAACCAGAGAAAGAGGAGTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11067.80 Da
• Isoelectric Point: 6.2312

>AT44416 WP_071196363.1 NZ_AP028047:1975629-1975931 [Phytobacter diazotrophicus]
MGRTLEQLLADEKPEVVAQAESMATETLLNIHLAELREKVQKTQVEMAHALGIRQPTVAGMEKPGRDVKLSTLKRYVEAT
GGKLRLDIELPDGSHYAFVV

Nucleotide

• Length: 303 bp

>AT44416 NZ_AP028047:1975629-1975931 [Phytobacter diazotrophicus]
ATGGGCAGAACGCTGGAACAACTGCTTGCGGATGAAAAACCAGAGGTTGTGGCGCAGGCTGAGTCAATGGCGACTGAGAC
TCTCCTGAACATTCACCTGGCCGAACTGCGTGAGAAAGTGCAAAAAACGCAGGTGGAAATGGCGCATGCACTGGGGATAC
GGCAACCTACGGTGGCGGGAATGGAAAAACCGGGTCGCGATGTAAAACTGTCGACGCTTAAGCGCTACGTTGAAGCCACT
GGCGGTAAGCTTCGTCTTGATATCGAACTGCCTGATGGGTCGCATTACGCCTTCGTTGTATAA

Structures

Antitoxin

• AlphaFold DB: A0A348DBJ5