Detailed information of TA system TA044396

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 1973737..1974395
• Replicon: chromosome
• Accession: NZ_AP028041
• Organism: Phytobacter diazotrophicus strain TA9730

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: QUE90_RS09235
• Protein ID: WP_125124251.1
• Coordinates: 1973737..1974090 (+)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A348DBJ5
• Locus tag: QUE90_RS09240
• Protein ID: WP_071196363.1
• Coordinates: 1974093..1974395 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUE90_RS09220 (1970191)	1970191..1971645	-	1455	WP_041851859.1	AMP nucleosidase	-
QUE90_RS09225 (1971839)	1971839..1972768	-	930	WP_232034419.1	MDR family oxidoreductase	-
QUE90_RS09230 (1972698)	1972698..1973518	-	821	Protein_1805	TetR family transcriptional regulator C-terminal domain-containing protein	-
QUE90_RS09235 (1973737)	1973737..1974090	+	354	WP_125124251.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
QUE90_RS09240 (1974093)	1974093..1974395	+	303	WP_071196363.1	XRE family transcriptional regulator	Antitoxin
QUE90_RS09245 (1974472)	1974472..1975422	+	951	WP_041851855.1	L,D-transpeptidase	-
QUE90_RS09250 (1975515)	1975515..1975703	-	189	WP_041851854.1	YjfB family protein	-
QUE90_RS09260 (1976301)	1976301..1977218	+	918	WP_041851853.1	nitrogen assimilation transcriptional regulator NAC	-
QUE90_RS09265 (1977320)	1977320..1978270	+	951	WP_041851852.1	HTH-type transcriptional regulator Cbl	-
QUE90_RS09270 (1978322)	1978322..1978798	-	477	WP_071196365.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1971839..2030267	58428

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-81)

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 13585.60 Da
• Isoelectric Point: 9.4816

>T44396 WP_125124251.1 NZ_AP028041:1973737-1974090 [Phytobacter diazotrophicus]
VWEIKTTDRFDEWLIAQNDHTRASVLAALIVLRTKGPELPRPWADTIKGARYSNMKELRIQCRGEPIRPFFAFDPRRAAI
VLCAGSKAGNEKRFYAEMISRAEQEYANYLKKPEKEE

Nucleotide

• Length: 354 bp

>T44396 NZ_AP028041:1973737-1974090 [Phytobacter diazotrophicus]
GTGTGGGAAATTAAAACAACGGACAGGTTTGATGAGTGGTTAATCGCTCAAAATGATCACACGCGTGCGAGCGTTTTGGC
GGCGCTGATCGTGTTGCGAACGAAAGGCCCCGAATTGCCCAGACCCTGGGCCGACACGATAAAAGGGGCGCGCTACAGCA
ATATGAAGGAGCTGCGAATTCAGTGTCGTGGGGAGCCGATACGCCCTTTTTTCGCTTTTGATCCCAGACGGGCGGCTATT
GTGTTGTGCGCAGGAAGTAAAGCTGGAAATGAAAAACGTTTCTACGCCGAAATGATTTCGCGAGCTGAGCAGGAATATGC
AAACTATCTGAAAAAACCAGAGAAAGAGGAGTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11067.80 Da
• Isoelectric Point: 6.2312

>AT44396 WP_071196363.1 NZ_AP028041:1974093-1974395 [Phytobacter diazotrophicus]
MGRTLEQLLADEKPEVVAQAESMATETLLNIHLAELREKVQKTQVEMAHALGIRQPTVAGMEKPGRDVKLSTLKRYVEAT
GGKLRLDIELPDGSHYAFVV

Nucleotide

• Length: 303 bp

>AT44396 NZ_AP028041:1974093-1974395 [Phytobacter diazotrophicus]
ATGGGCAGAACGCTGGAACAACTGCTTGCGGATGAAAAACCAGAGGTTGTGGCGCAGGCTGAGTCAATGGCGACTGAGAC
TCTCCTGAACATTCACCTGGCCGAACTGCGTGAGAAAGTGCAAAAAACGCAGGTGGAAATGGCGCATGCACTGGGGATAC
GGCAACCTACGGTGGCGGGAATGGAAAAACCGGGTCGCGATGTAAAACTGTCGACGCTTAAGCGCTACGTTGAAGCCACT
GGCGGTAAGCTTCGTCTTGATATCGAACTGCCTGATGGGTCGCATTACGCCTTCGTTGTATAA

Structures

Antitoxin

• AlphaFold DB: A0A348DBJ5