Detailed information of TA system TA044388

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 103445..103867
• Replicon: plasmid pVE-V11-M_1
• Accession: NZ_AP027979
• Organism: Escherichia coli strain VE-V11-M

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: QQK36_RS23035
• Protein ID: WP_223195199.1
• Coordinates: 103445..103567 (-)
• Length: 41 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	103659..103867 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QQK36_RS23000 (98510)	98510..98725	-	216	WP_001352842.1	conjugal transfer relaxosome protein TraY	-
QQK36_RS23005 (98861)	98861..99508	-	648	WP_000332520.1	conjugal transfer transcriptional regulator TraJ	-
QQK36_RS23010 (99700)	99700..100083	-	384	WP_001063020.1	conjugal transfer relaxosome DNA-binding protein TraM	-
QQK36_RS23015 (100423)	100423..101025	+	603	WP_077248356.1	transglycosylase SLT domain-containing protein	-
QQK36_RS23020 (101320)	101320..102141	-	822	WP_001234475.1	DUF932 domain-containing protein	-
QQK36_RS23025 (102260)	102260..102547	-	288	WP_000107520.1	hypothetical protein	-
QQK36_RS23030 (102847)	102847..103144	+	298	Protein_107	hypothetical protein	-
QQK36_RS23035 (103445)	103445..103567	-	123	WP_223195199.1	Hok/Gef family protein	Toxin
QQK36_RS23040 (103512)	103512..103625	-	114	Protein_109	DUF5431 family protein	-
- (103659)	103659..103867	-	209	NuclAT_0	-	Antitoxin
- (103659)	103659..103867	-	209	NuclAT_0	-	Antitoxin
- (103659)	103659..103867	-	209	NuclAT_0	-	Antitoxin
- (103659)	103659..103867	-	209	NuclAT_0	-	Antitoxin
QQK36_RS23045 (103812)	103812..104598	-	787	Protein_110	plasmid SOS inhibition protein A	-
QQK36_RS23050 (104595)	104595..105029	-	435	WP_000845922.1	conjugation system SOS inhibitor PsiB	-
QQK36_RS23055 (105084)	105084..107040	-	1957	Protein_112	ParB/RepB/Spo0J family partition protein	-
QQK36_RS23060 (107104)	107104..107335	-	232	Protein_113	DUF905 family protein	-
QQK36_RS23065 (107393)	107393..107932	-	540	WP_286611915.1	single-stranded DNA-binding protein	-
QQK36_RS23070 (107958)	107958..108164	-	207	WP_000547940.1	hypothetical protein	-
QQK36_RS23075 (108233)	108233..108657	+	425	Protein_116	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / blaTEM-1B / aac(3)-IId / dfrA14 / sitABCD	iroB / iroC / iroD / iroE / iroN / iutA / iucD / iucC / iucB / iucA	1..138038	138038

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (1-35)

Sequences

Toxin

Protein

• Length: 41 a.a.
• Molecular weight: 4536.35 Da
• Isoelectric Point: 8.2691

>T44388 WP_223195199.1 NZ_AP027979:c103567-103445 [Escherichia coli]
IVCCTLLIFTLLTRNRLCEVRLKDGYREVTASLAYESSGK

Nucleotide

• Length: 123 bp

>T44388 NZ_AP027979:c103567-103445 [Escherichia coli]
ATCGTGTGCTGCACGTTGTTAATATTCACACTCCTGACCCGGAATCGTCTGTGCGAAGTCCGGCTGAAGGACGGATACAG
GGAGGTTACGGCAAGTTTGGCTTACGAATCCAGCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 209 bp

>AT44388 NZ_AP027979:c103867-103659 [Escherichia coli]
TCACACGGATTACCCGTGAACTGGTTGAACGACCAGATTATTTTCAGGGAAAGTGAGTGTGGTCGTGCAGGGATATGAGC
TATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGGCAGA
AAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGCCCCCTGTATGTCT

Structures

Antitoxin