Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3595540..3596081 Replicon chromosome
Accession NZ_AP027978
Organism Escherichia coli strain VE-V11-M

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag QQK36_RS17695 Protein ID WP_000615983.1
Coordinates 3595803..3596081 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag QQK36_RS17690 Protein ID WP_000729703.1
Coordinates 3595540..3595800 (+) Length 87 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QQK36_RS17670 (3591121) 3591121..3591906 - 786 WP_000207589.1 putative lateral flagellar export/assembly protein LafU -
QQK36_RS17675 (3591878) 3591878..3593590 + 1713 Protein_3454 flagellar biosynthesis protein FlhA -
QQK36_RS17680 (3593908) 3593908..3594405 - 498 WP_000006256.1 REP-associated tyrosine transposase RayT -
QQK36_RS17685 (3594581) 3594581..3595330 - 750 WP_286611333.1 C40 family peptidase -
QQK36_RS17690 (3595540) 3595540..3595800 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
QQK36_RS17695 (3595803) 3595803..3596081 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
QQK36_RS17700 (3596237) 3596237..3596977 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
QQK36_RS17705 (3596948) 3596948..3597715 - 768 WP_000333380.1 class II glutamine amidotransferase -
QQK36_RS17710 (3597921) 3597921..3598499 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - gmhA/lpcA / vgrG/tssI / vgrG/tssI / hcp2/tssD2 / tssB / tssC / tssC 3589995..3627427 37432


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(4-91)

Antitoxin

(1-85)


Sequences


Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T44382 WP_000615983.1 NZ_AP027978:3595803-3596081 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T44382 NZ_AP027978:3595803-3596081 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT44382 WP_000729703.1 NZ_AP027978:3595540-3595800 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT44382 NZ_AP027978:3595540-3595800 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
PDB 4Q2U


Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References