Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1811185..1811687 Replicon chromosome
Accession NZ_AP027978
Organism Escherichia coli strain VE-V11-M

Toxin (Protein)


Gene name yoeB Uniprot ID D3GZ85
Locus tag QQK36_RS08795 Protein ID WP_000767820.1
Coordinates 1811433..1811687 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag QQK36_RS08790 Protein ID WP_001259255.1
Coordinates 1811185..1811436 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QQK36_RS08765 (1806362) 1806362..1807429 - 1068 WP_000080077.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
QQK36_RS08770 (1807429) 1807429..1808499 - 1071 WP_000108952.1 histidinol-phosphate transaminase -
QQK36_RS08775 (1808496) 1808496..1809800 - 1305 WP_039025710.1 histidinol dehydrogenase -
QQK36_RS08780 (1809806) 1809806..1810705 - 900 WP_284330258.1 ATP phosphoribosyltransferase -
QQK36_RS08785 (1810851) 1810851..1810901 - 51 WP_001364200.1 his operon leader peptide -
QQK36_RS08790 (1811185) 1811185..1811436 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
QQK36_RS08795 (1811433) 1811433..1811687 + 255 WP_000767820.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
QQK36_RS08800 (1811770) 1811770..1812594 + 825 WP_000754756.1 SDR family oxidoreductase -
QQK36_RS08805 (1812640) 1812640..1813569 + 930 WP_000803351.1 LysR substrate-binding domain-containing protein -
QQK36_RS08810 (1813784) 1813784..1813846 + 63 WP_010723108.1 membrane protein YoeI -
QQK36_RS08815 (1813836) 1813836..1815194 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
QQK36_RS08820 (1815373) 1815373..1816431 + 1059 WP_000492324.1 thiosulfate utilization transporter TsuA/YeeE -
QQK36_RS08825 (1816445) 1816445..1816672 + 228 WP_000985273.1 thiosulfate utilization sulfurtransferase TsuB/YeeD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10182.64 Da        Isoelectric Point: 8.0353

>T44370 WP_000767820.1 NZ_AP027978:1811433-1811687 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKIIELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T44370 NZ_AP027978:1811433-1811687 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CATTGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAGCATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT44370 WP_001259255.1 NZ_AP027978:1811185-1811436 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT44370 NZ_AP027978:1811185-1811436 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGCCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB D3GZ85

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References