Detailed information of TA system TA044356

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 118761..119182
• Replicon: plasmid pVE-V10-F_1
• Accession: NZ_AP027972
• Organism: Escherichia coli strain VE-V10-F

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: QQK73_RS24510
• Protein ID: WP_096937776.1
• Coordinates: 118761..118886 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	118984..119182 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QQK73_RS24470 (113826)	113826..114041	-	216	WP_001352842.1	conjugal transfer relaxosome protein TraY	-
QQK73_RS24475 (114177)	114177..114824	-	648	WP_000332520.1	conjugal transfer transcriptional regulator TraJ	-
QQK73_RS24480 (115016)	115016..115399	-	384	WP_001063020.1	conjugal transfer relaxosome DNA-binding protein TraM	-
QQK73_RS24485 (115739)	115739..116341	+	603	WP_077248356.1	transglycosylase SLT domain-containing protein	-
QQK73_RS24490 (116636)	116636..117457	-	822	WP_001234475.1	DUF932 domain-containing protein	-
QQK73_RS24495 (117576)	117576..117863	-	288	WP_000107546.1	hypothetical protein	-
QQK73_RS24500 (117888)	117888..118094	-	207	WP_000547965.1	hypothetical protein	-
QQK73_RS24505 (118164)	118164..118460	+	297	Protein_118	hypothetical protein	-
QQK73_RS24510 (118761)	118761..118886	-	126	WP_096937776.1	type I toxin-antitoxin system Hok family toxin	Toxin
QQK73_RS24515 (118828)	118828..118977	-	150	Protein_120	DUF5431 family protein	-
- (118984)	118984..119182	-	199	NuclAT_0	-	Antitoxin
- (118984)	118984..119182	-	199	NuclAT_0	-	Antitoxin
- (118984)	118984..119182	-	199	NuclAT_0	-	Antitoxin
- (118984)	118984..119182	-	199	NuclAT_0	-	Antitoxin
QQK73_RS24520 (119151)	119151..119913	-	763	Protein_121	plasmid SOS inhibition protein A	-
QQK73_RS24525 (119910)	119910..120344	-	435	WP_000845928.1	conjugation system SOS inhibitor PsiB	-
QQK73_RS24530 (120399)	120399..122357	-	1959	WP_029487628.1	ParB/RepB/Spo0J family partition protein	-
QQK73_RS24535 (122423)	122423..122527	-	105	Protein_124	DUF905 family protein	-
QQK73_RS24540 (122613)	122613..123358	-	746	Protein_125	IS21-like element ISEc12 family helper ATPase IstB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / dfrA14 / sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B / sitABCD	iroB / iroC / iroD / iroE / iroN / vat / iutA / iucD / iucC / iucB / iucA	1..169195	169195

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4806.72 Da
• Isoelectric Point: 8.4890

>T44356 WP_096937776.1 NZ_AP027972:c118886-118761 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T44356 NZ_AP027972:c118886-118761 [Escherichia coli]
GTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACGAAAATCGCTGTGCGAGATTCGTTACAGAGACGG
ATACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 199 bp

>AT44356 NZ_AP027972:c119182-118984 [Escherichia coli]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Antitoxin