Detailed information of TA system
Overview
TA module
| Type | I | Classification (family/domain) | hok-sok/- |
| Location | 88707..88961 | Replicon | plasmid pVE-V10-F_1 |
| Accession | NZ_AP027972 | ||
| Organism | Escherichia coli strain VE-V10-F | ||
Toxin (Protein)
| Gene name | srnB | Uniprot ID | G9G1E3 |
| Locus tag | QQK73_RS24340 | Protein ID | WP_001312851.1 |
| Coordinates | 88707..88856 (-) | Length | 50 a.a. |
Antitoxin (RNA)
| Gene name | srnC | ||
| Locus tag | - | ||
| Coordinates | 88900..88961 (+) |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| QQK73_RS24300 (84207) | 84207..85598 | - | 1392 | WP_001493761.1 | ISKra4-like element ISKpn19 family transposase | - |
| QQK73_RS24305 (85635) | 85635..86207 | - | 573 | WP_001493762.1 | recombinase family protein | - |
| QQK73_RS24310 (86344) | 86344..86889 | - | 546 | WP_001493763.1 | plasmid pRiA4b ORF-3 family protein | - |
| QQK73_RS24315 (87027) | 87027..87392 | - | 366 | Protein_80 | plasmid replication initiator RepA | - |
| QQK73_RS24320 (87385) | 87385..87867 | - | 483 | WP_001273588.1 | hypothetical protein | - |
| QQK73_RS24325 (87860) | 87860..87907 | - | 48 | WP_229471593.1 | hypothetical protein | - |
| QQK73_RS24330 (87898) | 87898..88149 | + | 252 | WP_223195197.1 | replication protein RepA | - |
| QQK73_RS24335 (88166) | 88166..88423 | - | 258 | WP_000083845.1 | replication regulatory protein RepA | - |
| QQK73_RS24340 (88707) | 88707..88856 | - | 150 | WP_001312851.1 | Hok/Gef family protein | Toxin |
| - (88900) | 88900..88961 | + | 62 | NuclAT_1 | - | Antitoxin |
| - (88900) | 88900..88961 | + | 62 | NuclAT_1 | - | Antitoxin |
| - (88900) | 88900..88961 | + | 62 | NuclAT_1 | - | Antitoxin |
| - (88900) | 88900..88961 | + | 62 | NuclAT_1 | - | Antitoxin |
| QQK73_RS24345 (89217) | 89217..89291 | - | 75 | Protein_86 | endonuclease | - |
| QQK73_RS24350 (89537) | 89537..89749 | - | 213 | WP_001299730.1 | ANR family transcriptional regulator | - |
| QQK73_RS24355 (89885) | 89885..90445 | - | 561 | WP_000139315.1 | fertility inhibition protein FinO | - |
| QQK73_RS24360 (90548) | 90548..91408 | - | 861 | WP_000704513.1 | alpha/beta hydrolase | - |
| QQK73_RS24365 (91467) | 91467..92213 | - | 747 | WP_284330307.1 | conjugal transfer pilus acetylase TraX | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | tet(A) / dfrA14 / sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B / sitABCD | iroB / iroC / iroD / iroE / iroN / vat / iutA / iucD / iucC / iucB / iucA | 1..169195 | 169195 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 50 a.a. Molecular weight: 5542.67 Da Isoelectric Point: 8.7678
>T44353 WP_001312851.1 NZ_AP027972:c88856-88707 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK
Download Length: 150 bp
>T44353 NZ_AP027972:c88856-88707 [Escherichia coli]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAGCTGAATATTCACAGAGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAGCTGAATATTCACAGAGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA
Antitoxin
Download Length: 62 bp
>AT44353 NZ_AP027972:88900-88961 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
