Detailed information of TA system TA044336

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1118786..1119513
• Replicon: chromosome
• Accession: NZ_AP027971
• Organism: Escherichia coli strain VE-V10-F

Toxin (Protein)

• Gene name: higB
• Uniprot ID: J7Q991
• Locus tag: QQK73_RS05535
• Protein ID: WP_000547564.1
• Coordinates: 1118786..1119097 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: QQK73_RS05540
• Protein ID: WP_000126294.1
• Coordinates: 1119094..1119513 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QQK73_RS05505 (1113928)	1113928..1115637	+	1710	WP_001288134.1	formate hydrogenlyase subunit HycE	-
QQK73_RS05510 (1115647)	1115647..1116189	+	543	WP_000493792.1	formate hydrogenlyase subunit HycF	-
QQK73_RS05515 (1116189)	1116189..1116956	+	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
QQK73_RS05520 (1116953)	1116953..1117363	+	411	WP_001291921.1	formate hydrogenlyase assembly protein HycH	-
QQK73_RS05525 (1117356)	1117356..1117826	+	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
QQK73_RS05530 (1117851)	1117851..1118612	+	762	WP_001026446.1	hypothetical protein	-
QQK73_RS05535 (1118786)	1118786..1119097	+	312	WP_000547564.1	type II toxin-antitoxin system HigB family toxin	Toxin
QQK73_RS05540 (1119094)	1119094..1119513	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
QQK73_RS05545 (1119627)	1119627..1121051	-	1425	WP_000110363.1	6-phospho-beta-glucosidase AscB	-
QQK73_RS05550 (1121060)	1121060..1122517	-	1458	WP_284329962.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
QQK73_RS05555 (1122777)	1122777..1123787	+	1011	WP_001392554.1	DNA-binding transcriptional regulator AscG	-
QQK73_RS05560 (1123936)	1123936..1124463	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12426.13 Da
• Isoelectric Point: 9.7248

>T44336 WP_000547564.1 NZ_AP027971:1118786-1119097 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T44336 NZ_AP027971:1118786-1119097 [Escherichia coli]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAGATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT44336 WP_000126294.1 NZ_AP027971:1119094-1119513 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT44336 NZ_AP027971:1119094-1119513 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAGTATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCGGTT
GGCGTGGCGTTACTTCGCACGTTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A0A0FJG5