Detailed information of TA system TA044294

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4691497..4692099
• Replicon: chromosome
• Accession: NZ_AP027960
• Organism: Escherichia coli strain VE-T2

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: QQK13_RS22815
• Protein ID: WP_000897305.1
• Coordinates: 4691788..4692099 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: QQK13_RS22810
• Protein ID: WP_000356397.1
• Coordinates: 4691497..4691787 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QQK13_RS22785 (4687442)	4687442..4688344	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
QQK13_RS22790 (4688341)	4688341..4688976	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
QQK13_RS22795 (4688973)	4688973..4689902	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
QQK13_RS22800 (4690232)	4690232..4690474	-	243	WP_001087409.1	protein YiiF	-
QQK13_RS22805 (4690693)	4690693..4690911	-	219	WP_001295676.1	CopG family transcriptional regulator	-
QQK13_RS22810 (4691497)	4691497..4691787	-	291	WP_000356397.1	NadS family protein	Antitoxin
QQK13_RS22815 (4691788)	4691788..4692099	-	312	WP_000897305.1	hypothetical protein	Toxin
QQK13_RS22820 (4692328)	4692328..4693236	+	909	WP_001162704.1	alpha/beta hydrolase	-
QQK13_RS22825 (4693300)	4693300..4694241	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
QQK13_RS22830 (4694286)	4694286..4694723	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
QQK13_RS22835 (4694720)	4694720..4695592	-	873	WP_000920762.1	virulence factor BrkB family protein	-
QQK13_RS22840 (4695586)	4695586..4696185	-	600	WP_001295269.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T44294 WP_000897305.1 NZ_AP027960:c4692099-4691788 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T44294 NZ_AP027960:c4692099-4691788 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT44294 WP_000356397.1 NZ_AP027960:c4691787-4691497 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT44294 NZ_AP027960:c4691787-4691497 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7